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Purified Mouse Anti- AIB-1
Purified Mouse Anti- AIB-1

Western blot analysis of AIB-1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- AIB-1 antibody.

Western blot analysis of AIB-1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- AIB-1 antibody.

Product Details
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BD Transduction Laboratories™
Amplified In Breast cancer-1; pCIP; RAC3; TRAM-1
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human AIB-1 aa. 376-389
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
160 kDa
250 µg/ml
AB_2151198
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611104 Rev. 3
Antibody Details
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34/AIB-1

Signal transduction via nuclear hormone receptors is important for cell growth and differentiation, development, and homeostasis. Nuclear hormone receptors are ligand-activated transcription factors that modulate target gene expression. These ligand/receptor complexes also interact with transcriptional coactivators which enhance ligand-dependent transcription. Various classes of coactivators have been identified, including SRC-1 and its related proteins, such as TIF-2/GRIP-1, RIP140, AIB-1, and TIF-1α and -1β. AIB-1 (Amplified In Breast cancer-1) is also known as pCIP, RAC3, and TRAM-1. It interacts with estrogen receptor (ER) and is overexpressed in breast cancer biopsies and several breast and ovarian cancer cell lines. Similar to SRC-1 and TIF2, AIB-1 contains a basic helix-loop-helix (bHLH) domain followed by a PAS (Per/Arnt/Sim) region, serine and threonine rich regions, and a charged cluster. In addition, AIB-1 contains three copies of the conserved LXXLL motif which is critical to its interaction with the nuclear receptor. Thus, AIB-1 is a coactivator of nuclear receptors that may participate in the development of steroid-dependent cancers.

611104 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611104 Rev.3
Citations & References
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Development References (5)

  1. Anzick SL, Kononen J, Walker RL, et al. AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer. Science. 1997; 277(5328):965-968. (Biology). View Reference
  2. Eng FC, Barsalou A, Akutsu N, et al. Different classes of coactivators recognize distinct but overlapping binding sites on the estrogen receptor ligand binding domain. J Biol Chem. 1998; 273(43):28371-28377. (Biology). View Reference
  3. Lauritsen KJ, List HJ, Reiter R, Wellstein A, Riegel AT. A role for TGF-beta in estrogen and retinoid mediated regulation of the nuclear receptor coactivator AIB1 in MCF-7 breast cancer cells. Oncogene. 2002; 21(47):7147-7155. (Biology: Western blot). View Reference
  4. Louie MC, Yang HQ, Ma AH, et al. Androgen-induced recruitment of RNA polymerase II to a nuclear receptor-p160 coactivator complex. Proc Natl Acad Sci U S A. 2003; 100(5):2226-2230. (Biology: Western blot). View Reference
  5. Reiter R, Wellstein A, Riegel AT. An isoform of the coactivator AIB1 that increases hormone and growth factor sensitivity is overexpressed in breast cancer. J Biol Chem. 2001; 276(43):39736-39741. (Biology: Western blot). View Reference
View All (5) View Less
611104 Rev. 3

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.