Skip to main content Skip to navigation
Biotin Mouse Anti-SV40 Large T and Small t Antigens
Biotin Mouse Anti-SV40 Large T and Small t Antigens

Immunohistochemical staining of SV40 large T, small t antigen. Cytospins of SV40 transformed COS-7 cells were acetone fixed and stained with mouse anti-SV40 largeT, small t antigen (Cat.No. 554151) (left panel) or with an isotype control (right panel). SV40 T-ag staining is localized to the nucleus of the cells.

Immunohistochemical staining of SV40 large T, small t antigen. Cytospins of SV40 transformed COS-7 cells were acetone fixed and stained with mouse anti-SV40 largeT, small t antigen (Cat.No. 554151) (left panel) or with an isotype control (right panel). SV40 T-ag staining is localized to the nucleus of the cells.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
Viral (QC Testing)
Mouse IgG2a
SV40-transformed BALB/c mouse cells
Immunofluorescence (Routinely Tested), Immunohistochemistry, Immunoprecipitation, Western blot (Tested During Development)
90-100 kDa
0.5 mg/ml
AB_395271
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

By SDS-PAGE, T-ag migrates at 90-100 kDa and t-ag at 15-20 kDa. COS-7 monkey kidney cells (ATCC CRL-1651), other established SV40-transformed cell lines, SV40-virus infected cells, or cells transfected with SV40 DNA should be used as a positive control.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554151 Rev. 7
Antibody Details
Down Arrow Up Arrow
PAb 108

Simian virus 40 (SV40) is a small DNA virus encoded by 5.2 kb of double-stranded DNA. SV40 large T-antigen (T-ag) is a multifunctional ~85 kDa phosphoprotein, which is the sole viral protein required for SV40 replication. All other factors are provided by the infected host cell. In addition to its role in SV40 DNA replication, T-ag also causes transformation of susceptible cell lines. Studies of various mutant T-ag proteins have shown that the replication and transformation fractions of T-ag can be separated. The multifunctional nature of this protein has resulted in its use as a model system in a wide variety of disciplines. T-ag exercises negative regulation on the transcription of SV40 early mRNA by feedback inhibition and exerts positive regulation on transcription from the late promoter. In addition to transcriptional regulation, T-ag is involved in viral DNA replication. Specific biochemical functions required for DNA synthesis that are inherent to the T-ag include high-affinity binding to sites within the viral origin of DNA synthesis, ATPase, and helicase activities. Other functions attributed to T-ag include cellular transformation, induction of cellular DNA synthesis, induction of rRNA synthesis, and provision of a host-range function for viral replication. However, functions of T-ag are influenced by a wide range of post-translational modifications including phosphorylation, glycosylation, acetylation, acylation, and adenylation. T-ag exists in monomeric as well as polymeric forms, and associates with the tumor suppressor proteins p53 and retinoblastoma protein (Rb). Most of T-ag is transported to the nucleus, while a small fraction is localized at the cell surface. Small t-Ag is a polypeptide which shares 82 N-terminal amino acids with large T antigen and has a unique C-terminal region.

Clone PAb 108 recognizes an N-terminal epitope within the first 82 amino acids of T-ag and small t antigen (t-ag). B4 SV40-transformed BALB/c mouse fibroblasts were used as immunogen. PAb 108 was originally produced and characterized as part of a panel of antibodies designated PAb 102-117.

554151 Rev. 7
Format Details
Down Arrow Up Arrow
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
554151 Rev.7
Citations & References
Down Arrow Up Arrow

Development References (3)

  1. Gurney EG, Tamowski S, Deppert W. Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen. J Virol. 1986; 57(3):1168-1172. (Immunogen: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  2. Hinzpeter M, Fanning E, Deppert W. A new sensitive target-bound DNA binding assay for SV40 large T antigen. Virology. 1986; 148(1):159-167. (Clone-specific: Immunohistochemistry, Immunoprecipitation). View Reference
  3. Mellor A, Smith AE. Characterization of the amino-terminal tryptic peptide of simian virus 40 small-t and large-T antigens. J Virol. 1978; 28(3):992-996. (Biology). View Reference
554151 Rev. 7

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.