Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- CF™ is a trademark of Biotium, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Companion Products
The MR5-2 antibody reacts with the Vβ 8.1 and Vβ 8.2 T-cell Receptors (TCR), but not the Vβ 8.3 TCR, of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V8 subfamily gene loci are deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype. Vβ 8.1 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by the Mtv-7 (Mls-1a, Mlsa), provirus (e.g., AKR, CBA/J, C58, DBA/2), and activation or elimination of Vβ 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E. Mtv-43 (e.g., MA/MyJ), Mtv-44 (e.g., NZW), and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ 8.1 TCR-bearing T cells. In addition to expression on conventional T lymphocytes, Vβ 8.2 is the predominant β chain of the TCR on NK-T cells. Staphylococcal enterotoxin B, in association with antigen presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing Vβ 8 TCR and selectively eliminates those T cells in vivo. Plate-bound MR5-2 antibody activates Vβ 8.1 or 8.2 TCR-bearing T lymphocytes.
Development References (11)
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Kanagawa O. Antibody-mediated activation of T cell clones as a method for screening hybridomas producing antibodies to the T cell receptor. J Immunol Methods. 1988; 110(2):169-178. (Immunogen). View Reference
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Shinohara K, Ikarashi Y, Maruoka H, et al. Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains. Eur J Immunol. 1999; 29(6):1871-1878. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.