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RB780 Mouse Anti-Human CD1c
RB780 Mouse Anti-Human CD1c
Multiparameter flow cytometric analysis of CD1c expression on Human peripheral blood leucocytes. Human whole blood was stained with APC Mouse Anti-Human CD19 antibody (Cat. No. 555415) and with either BD Horizon™ RB780 Mouse IgG1, κ Isotype Control (Cat. No. 568532; Left Plots) or BD Horizon™ RB780 Mouse Anti-Human CD1c antibody (Cat. No. 569337/569338; Right Plots). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plots showing the correlated expression of CD1c [or Ig Isotype control staining] versus CD19 (Top Plots) or side light-scatter (SSC-A) signals (Bottom Plots) were derived from gated events with the forward and side light-scatter characteristics of either viable lymphocytes or leucocyte populations, respectively. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Multiparameter flow cytometric analysis of CD1c expression on Human peripheral blood leucocytes. Human whole blood was stained with APC Mouse Anti-Human CD19 antibody (Cat. No. 555415) and with either BD Horizon™ RB780 Mouse IgG1, κ Isotype Control (Cat. No. 568532; Left Plots) or BD Horizon™ RB780 Mouse Anti-Human CD1c antibody (Cat. No. 569337/569338; Right Plots). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plots showing the correlated expression of CD1c [or Ig Isotype control staining] versus CD19 (Top Plots) or side light-scatter (SSC-A) signals (Bottom Plots) were derived from gated events with the forward and side light-scatter characteristics of either viable lymphocytes or leucocyte populations, respectively. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Product Details
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BD Horizon™
CD1; R7; M241; BDCA1
Human (QC Testing)
Mouse IgG1, κ
GM-CSF- and IL-4-activated Human Monocytes
Flow cytometry (Routinely Tested)
5 µl/test
911
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  7. An isotype control should be used at the same concentration as the antibody of interest.
  8. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  10. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
  11. For U.S. patents that may apply, see bd.com/patents.
569338 Rev. 2
Antibody Details
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F10/21A3

The F10/21A3 monoclonal antibody specifically binds to CD1c. The CD1 family of transmembrane glycoproteins are structurally related to the classical major histocompatibility complex (MHC) proteins. CD1c is a type I transmembrane glycoprotein that forms heterodimers with beta-2-microglobulin. CD1c presents lipids and glycolipids of self or microbial origin to T cells. CD1c is expressed by Langerhans cells, dendritic cells, monocytes, cortical thymocytes, T cells, and some B cells.

569338 Rev. 2
Format Details
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RB780
The BD Horizon RealBlue™ 780 (RB780) Dye is part of the BD family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 498-nm and an emission maximum (Em Max) at 781-nm. Driven by BD innovation, RB780 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with minimal excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), RB780 can be used as an alternative to PE-Cy7 and we recommend using an optical filter centered near 780-nm (eg, a 780/60-nm bandpass filter). For spectral instruments equipped with a Blue laser (488-nm), it can be used in conjunction with PE-Cy7. RB780 is on average brighter than PE-Cy7 and has minimal spillover into Yellow-Green detectors.
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RB780
Blue 488 nm
498 nm
781 nm
569338 Rev.2
Citations & References
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View product citations for antibody "569338" on CiteAb

Development References (4)

  1. Delia D, Cattoretti G, Polli N, et al. CD1c but neither CD1a nor CD1b molecules are expressed on normal, activated, and malignant human B cells: identification of a new B-cell subset. Blood. 1988; 72(1):241-247. (Biology). View Reference
  2. Grant EP, Degano M, Rosat JP, et al. Molecular recognition of lipid antigens by T cell receptors. J Exp Med. 1999; 189(1):195-205. (Clone-specific: Blocking, Functional assay, Inhibition). View Reference
  3. Melian A, Geng YJ, Sukhova GK, Libby P, Porcelli SA. CD1 expression in human atherosclerosis. A potential mechanism for T cell activation by foam cells. Am J Pathol. 1999; 155(3):775-786. (Immunogen: Flow cytometry). View Reference
  4. Moody DB, Ulrichs T, Mühlecker W, et al. CD1c-mediated T-cell recognition of isoprenoid glycolipids in Mycobacterium tuberculosis infection. Nature. 2000; 404(6780):884-888. (Clone-specific: Blocking, Functional assay, Inhibition). View Reference
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569338 Rev. 2

 

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.