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Purified Rat Anti-Mouse Panendothelial Cell Antigen
Purified Rat Anti-Mouse Panendothelial Cell Antigen

Immunohistochemical staining of mouse panendothelial cell antigen. Frozen sections of normal mouse small intestine were reacted with Purified Rat Anti-Mouse Panendothelial Cell Antigen (Cat. No. 550563). Visualization was carried out with Biotin Goat Anti-Rat Ig (Cat. No. 559286), Streptavidin-HRP (Cat. No. 550946), and DAB detection system (Cat. No. 550880). Cells expressing this molecule can be identified by the brown labeling of their cell membranes. Amplification 20X.

Immunohistochemical staining of mouse panendothelial cell antigen. Frozen sections of normal mouse small intestine were reacted with Purified Rat Anti-Mouse Panendothelial Cell Antigen (Cat. No. 550563). Visualization was carried out with Biotin Goat Anti-Rat Ig (Cat. No. 559286), Streptavidin-HRP (Cat. No. 550946), and DAB detection system (Cat. No. 550880). Cells expressing this molecule can be identified by the brown labeling of their cell membranes. Amplification 20X.

Product Details
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BD Pharmingen™
Plvap; Pv1; MECA32; Plasmalemma vesicle-associated protein
Mouse (QC Testing)
Rat IgG2a, κ
Mouse lymph node stromal cells
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
31.25 µg/ml
84094
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Immunohistochemistry: The MECA-32 antibody specific for mouse panendothelial cell antigen is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were mouse spleen, thymus and small intestine. The antibody stains endothelial cells. The isotype control recommended for use with this antibody is Purified Rat IgG2a κ Isotype Control (Cat. No. 559073). For optimal indirect immunohistochemical staining, the MECA-32 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with Biotin Goat Anti-Rat Ig (Cat. No. 559286) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). A detailed protocol of the immunohistochemical procedure is available on our website, http://www.bdbiosciences.com/us/s/resources, under "Cell Biology (WB, IP, IHC, IF)".

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550563 Rev. 3
Antibody Details
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MECA-32

The MECA-32 antibody reacts with a dimer of 50-55–kDa subunits expressed on most or all endothelial cells in the embryonic and adult mouse, with the exception of cardiac and skeletal muscle and the brain. Normally in skeletal and cardiac muscle, MECA-32 antigen expression is limited to small arterioles and venules; however, under conditions of inflammation, it can be induced on previously non-expressing vessels in cardiac muscle. In the central nervous system (CNS), the panendothelial cell antigen expression is developmentally regulated. During embryonic development, the antigen is found on brain vasculature up to day 16 of gestation, after which it disappears. The cessation of MECA-32 antigen expression in the CNS may be associated with the establishment of the blood-brain barrier, which begins on day 16 of gestation. In the adult mouse,  inflammation in the CNS can lead to re-expression of the panendothelial cell antigen.

550563 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550563 Rev.3
Citations & References
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Development References (5)

  1. Bergese SD, Pelletier RP, Ohye RG, Vallera DA, Orosz CG. Treatment of mice with anti-CD3 mAb induces endothelial vascular cell adhesion molecule-1 expression. Transplantation. 1994; 57(5):711-717. (Biology). View Reference
  2. Engelhardt B, Conley FK, Butcher EC. Cell adhesion molecules on vessels during inflammation in the mouse central nervous system. J Neuroimmunol. 1994; 51(2):199-208. (Biology). View Reference
  3. Hallmann R, Mayer DN, Berg EL, Broermann R, Butcher EC. Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier. Dev Dyn. 1995; 202(4):325-332. (Biology). View Reference
  4. Leppink DM, Bishop DK, Sedmak DD, et al. Inducible expression of an endothelial cell antigen on murine myocardial vasculature in association with interstitial cellular infiltration. Transplantation. 1989; 48(5):874-877. (Immunogen). View Reference
  5. Orosz CG, van Buskirk A, Sedmak DD, Kincade P, Miyake K, Pelletier RP. Role of the endothelial adhesion molecule VCAM in murine cardiac allograft rejection. Immunol Lett. 1992; 32(1):7-12. (Biology). View Reference
View All (5) View Less
550563 Rev. 3

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.