BD Pharmingen™ Purified Rat Anti-Mouse CD206
Clone Y17-505 (RUO)
Two-color flow cytometric analysis of CD206 expression on Mouse Thioglycolate-elicited Peritoneal Exudate Cells (Thio-PECs). C57BL/6 Mouse Thio-PECs were stained with Purified Rat IgG2a, κ Isotype Control (Cat. No. 555841) or Purified Rat Anti-Mouse CD206 antibody (Cat. No. 568839) at 1.0 μg/test. The cells were washed and secondarily stained with PE Goat Anti-Rat Ig (Cat. No. 550767). The cells were then blocked with purified normal Rat IgG (Rat IgG Isotype Control, Thermo Fisher Cat. No. 10700) before staining with Alexa Fluor™ 647 Rat Anti-Mouse CD107b antibody (Cat. No. 564843). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 (or Ig Isotype control staining) versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) Thio-PECs. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Two-color flow cytometric analysis of CD206 expression on Mouse Thioglycolate-elicited Peritoneal Exudate Cells (Thio-PECs). C57BL/6 Mouse Thio-PECs were stained with Purified Rat IgG2a, κ Isotype Control (Cat. No. 555841) or Purified Rat Anti-Mouse CD206 antibody (Cat. No. 568839) at 1.0 μg/test. The cells were washed and secondarily stained with PE Goat Anti-Rat Ig (Cat. No. 550767). The cells were then blocked with purified normal Rat IgG (Rat IgG Isotype Control, Thermo Fisher Cat. No. 10700) before staining with Alexa Fluor™ 647 Rat Anti-Mouse CD107b antibody (Cat. No. 564843). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 (or Ig Isotype control staining) versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) Thio-PECs. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Preparation And Storage
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Companion Products
The Y17-505 monoclonal antibody specifically binds to CD206 which is also known as the Macrophage mannose receptor (MMR, MR) or Mannose receptor, C type 1 (Mrc1). CD206 is a type I transmembrane glycoprotein of approximately 175 kDa that belongs to the C-type lectin superfamily. It is expressed at the cell surface and intracellularly by macrophages, Langerhans cells, dendritic cells, and endothelial cells within hepatic and lymphoid tissues. This pattern recognition receptor binds to endogenous and microbial glycoconjugates containing mannose, fucose, or N-acetylglucosamine through its C-type lectin-like carbohydrate recognition domains (CRD). CD206 also contains a cysteine-rich domain that enables binding to sulfated carbohydrate antigens. This receptor enables macrophages and other specialized cells to maintain tissue homeostasis as well as to internalize microbes or their components by phagocytosis or endocytosis. CD206 thus plays important roles in mediating innate immunity, eg, enabling phagocytosis, as well as in processing and presenting antigens for the generation and expression of adaptive immunity. Moreover, CD206 has been associated with leucocyte homing and cancer cell metastasis.
Development References (6)
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Akbarshahi H, Menzel M, Posaric Bauden M, Rosendahl A, Andersson R. Enrichment of murine CD68+ CCR2+ and CD68+ CD206+ lung macrophages in acute pancreatitis-associated acute lung injury. PLoS ONE. 2012; 7(10):e42654. (Biology). View Reference
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Burgdorf S, Lukacs-Kornek V, Kurts C. The mannose receptor mediates uptake of soluble but not of cell-associated antigen for cross-presentation. J Immunol. 2006; 176(11):6770-6776. (Biology). View Reference
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Marttila-Ichihara F, Turja R, Miiluniemi M, et al. Macrophage mannose receptor on lymphatics controls cell trafficking. Blood. 2008; 112(1):64-72. (Biology). View Reference
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McKenzie EJ, Taylor PR, Stillion RJ, et al. J Immunol. 2007; 178(8):4975-4983. (Biology). View Reference
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Rybalko V, Hsieh PL, Merscham-Banda M, Suggs LJ, Farrar RP. The Development of Macrophage-Mediated Cell Therapy to Improve Skeletal Muscle Function after Injury.. PLoS One. 2015; 10(12):e0145550. (Biology). View Reference
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Zamze S, Martinez-Pomares L, Jones H, et al. Recognition of bacterial capsular polysaccharides and lipopolysaccharides by the macrophage mannose receptor. J Biol Chem. 2002; 277(44):41613-41623. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.