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Purified Mouse P-Selectin - IgG Fusion Protein
Purified Mouse P-Selectin - IgG Fusion Protein

Detection of P-Selectin ligands on mouse granulocytes (left panel) and mononuclear cells (right panel). BALB/c whole blood was stained with Mouse P-Selectin - IgG Fusion Protein followed by R-PE-conjugated goat anti-human IgG, Fcγ fragment specific (Jackson Immunoresearch, open histogram) or with the second-step alone (filled histogram). Erythrocytes were lysed (BD Pharm Lyse™ lysis buffer, Cat. No. 555899), non-viable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™ cell viability dye, Cat. No. 555815/555816), and granulocytes and mononuclear cells were gated according to their light-scatter profiles. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Detection of P-Selectin ligands on mouse granulocytes (left panel) and mononuclear cells (right panel). BALB/c whole blood was stained with Mouse P-Selectin - IgG Fusion Protein followed by R-PE-conjugated goat anti-human IgG, Fcγ fragment specific (Jackson Immunoresearch, open histogram) or with the second-step alone (filled histogram). Erythrocytes were lysed (BD Pharm Lyse™ lysis buffer, Cat. No. 555899), non-viable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™ cell viability dye, Cat. No. 555815/555816), and granulocytes and mononuclear cells were gated according to their light-scatter profiles. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Product Details
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BD Pharmingen™
Mouse (QC Testing)
IgG1
Flow cytometry (Routinely Tested), Immunoprecipitation (Reported)
0.5 mg/ml
AB_10167545
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Avoid multiple freeze-thaws of product.

The fusion protein solution should be stored at -20˚C until the vial is opened. After opening, undiluted aliquots should be stored at -20˚C or below. Thawed aliquots may be stored up to two months at 4˚C.

Recommended Assay Procedures

For detection of the fusion protein, we recommend a polyclonal anti-hman Ig second step. Other reported applications include affinity isolation of P-selectin ligand.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
555294 Rev. 8
Antibody Details
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p-Selectin-Ig CHO

The Mouse P-selectin - IgG Fusion Protein is composed of the signal sequence, the lectin domain, the EGF-like repeat, and the first two complement-binding domains of mouse P-selectin (CD62P) fused to the Fc region (hinge, CH2 and CH3) of human IgG1. This chimeric protein binds to P-selectin ligand expressed on mouse neutrophils and myeloid cell lines. Furthermore, P-selectin-IgG fusion protein selectively stains Th1 cells, but not Th2 cells.reported in the literature.

555294 Rev. 8
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555294 Rev.8
Citations & References
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Development References (4)

  1. Austrup F, Vestweber D, Borges E, et al. P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues. Nature. 1997; 385(6611):81-83. (Biology). View Reference
  2. Hahne M, Jager U, Isenmann S, Hallmann R, Vestweber D. Five tumor necrosis factor-inducible cell adhesion mechanisms on the surface of mouse endothelioma cells mediate the binding of leukocytes.. J Cell Biol. 1993; 121(3):655-664. (Biology). View Reference
  3. Katakai T, Mori KJ, Masuda T, Shimizu A. Selective accumulation of type 1 effector cells expressing P-selectin ligand and/or alpha(4)beta(7)-integrin at the lesions of autoimmune gastritis. Int Immunol. 2002; 14(2):167-175. (Biology). View Reference
  4. Lenter M, Levinovitz A, Isenmann S, Vestweber D. Monospecific and common glycoprotein ligands for E- and P-selectin on myeloid cells. J Cell Biol. 1994; 125(2):471-481. (Biology). View Reference
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555294 Rev. 8

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.