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Purified Mouse Anti-Rat Granulocytes
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This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [550000] or for additional support, contact your local applications specialist. Contact Us #
Product Details
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BD Pharmingen™
Rat (QC Testing)
Mouse IgM, κ
Not reported
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-zinc-fixed (Reported)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Other applications include immunohistochemical staining of acetone-fixed frozen, formalin- or zinc-fixed paraffin-embedded, and plasticembedded sections.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554905 Rev. 11
Antibody Details
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The HIS48 antibody reacts with an antigen found on all granulocytes. It is also found on cells of the erythroid lineage at various stages of maturation in bone marrow.

554905 Rev. 11
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
554905 Rev.11
Citations & References
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Development References (4)

  1. Badger DA, Sauer JM, Hoglen NC, Jolley CS, Sipes IG. The role of inflammatory cells and cytochrome P450 in the potentiation of CCl4-induced liver injury by a single dose of retinol. Toxicol Appl Pharmacol. 1996; 141(2):507-519. (Clone-specific: Immunohistochemistry). View Reference
  2. Kudo C, Araki A, Matsushima K, Sendo F. Inhibition of IL-8-induced W3/25+ (CD4+) T lymphocyte recruitment into subcutaneous tissues of rats by selective depletion of in vivo neutrophils with a monoclonal antibody. J Immunol. 1991; 147(7):2196-2201. (Clone-specific: Depletion). View Reference
  3. Sekiya S, Gotoh S, Yamashita T, Watanabe T, Saitoh S, Sendo F. Selective depletion of rat neutrophils by in vivo administration of a monoclonal antibody. J Leukoc Biol. 1989; 46(2):96-102. (Clone-specific: Depletion). View Reference
  4. van Goor H, Fidler V, Weening JJ, Grond J. Determinants of focal and segmental glomerulosclerosis in the rat after renal ablation. Evidence for involvement of macrophages and lipids. Lab Invest. 1991; 64(6):754-765. (Clone-specific: Immunohistochemistry). View Reference
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554905 Rev. 11

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.