Reacts with NPM-ALK, a chimeric gene product of approximately 80 kDa, which is the result of t(2;5)(p23;q35) chromosomal translocation. The gene at 5q35 encodes for the cell cycle-regulated nuclolar protein nucleophosmin (NPM), and the gene at 2p23 encodes a novel receptor tyrosine kinase, anaplastic lymphoma kinase (ALK). The 2;5 translocation results in the expression of NPM-ALK protein, or p80, comprised of the N-terminal portion of NPM (about 40% of the molecule) linked to the entire intracellular portion of ALK. ALK1 antibody recognizes a formalin resistant epitope in both the NPM-ALK chimeric and the 200 kDa normal human ALK proteins. Cytoplasmic and nuclear expression of NPM-ALK is seen in t(2;5)+ cell lines SU-DHL-1 and Karpas 299.
This antibody is routinely tested by flow cytometric analysis and fluorescence microscopy. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.