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      Purified Mouse Anti-Human NPM-ALK/ALK
      Product Details
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      BD Pharmingen™
      Human (QC Testing)
      Mouse IgG3, κ
      Intracellular staining (flow cytometry) (Routinely Tested), Fluorescence microscopy, Immunohistochemistry-paraffin (Tested During Development)
      0.5 mg/ml
      AB_397207
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Recommended Assay Procedures

      We recommend to use the Cytofix/cytoperm solution (Cat. No. 554714) for the cell fixation and permeabilization. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      559254 Rev. 5
      Antibody Details
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      ALK1

      Reacts with NPM-ALK, a chimeric gene product of approximately 80 kDa, which is the result of t(2;5)(p23;q35) chromosomal translocation. The gene at 5q35 encodes for the cell cycle-regulated nuclolar protein nucleophosmin (NPM), and the gene at 2p23 encodes a novel receptor tyrosine kinase, anaplastic lymphoma kinase (ALK). The 2;5 translocation results in the expression of NPM-ALK protein, or p80, comprised of the N-terminal portion of NPM (about 40% of the molecule) linked to the entire intracellular portion of ALK. ALK1 antibody recognizes a formalin resistant epitope in both the NPM-ALK chimeric and the 200 kDa normal human ALK proteins. Cytoplasmic and nuclear expression of NPM-ALK is seen in t(2;5)+ cell lines SU-DHL-1 and Karpas 299.

      This antibody is routinely tested by flow cytometric analysis and fluorescence microscopy. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      559254 Rev. 5
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      559254 Rev.5
      Citations & References
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      Development References (3)

      1. Morris SW, Kirstein MN, Valentine MB, et al. Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin's lymphoma. Science. 1994; 263(5151):1281-1284. (Biology). View Reference
      2. Pittaluga S, Wlodarska I, Pulford K, et al. The monoclonal antibody ALK1 identifies a distinct morphological subtype of anaplastic large cell lymphoma associated with 2p23/ALK rearrangements. Am J Pathol. 1997; 151(2):343-351. (Biology). View Reference
      3. Pulford K, Lamant L, Morris SW, et al. Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1. Blood. 1997; 89(4):1394-1404. (Biology). View Reference
      559254 Rev. 5

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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