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Purified Mouse Anti-Human c-erbB-2
Purified Mouse Anti-Human c-erbB-2

Flow cytometric analysis of c-erbB-2 expression on SK-BR-3 (human breast carcinoma) cell line. SK-BR-3 (ATCC HTB-30) cells were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 555648; dashed line histogram) or Purified Mouse Anti-Human c-erbB-2 (Cat. No. 554300; solid line histogram), followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). Fluorescent histograms are derived from gated events with the forward and side light-scattering characteristics of viable cells.

Flow cytometric analysis of c-erbB-2 expression on SK-BR-3 (human breast carcinoma) cell line. SK-BR-3 (ATCC HTB-30) cells were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 555648; dashed line histogram) or Purified Mouse Anti-Human c-erbB-2 (Cat. No. 554300; solid line histogram), followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). Fluorescent histograms are derived from gated events with the forward and side light-scattering characteristics of viable cells.

Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1
Recombinant human c-erbB-2
Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development), Immunoprecipitation (Reported)
0.5 mg/ml
AB_395353
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Positive control cells lines include MCF7 (ATCC HTB 22) and SK-BR-3 (ATCC HTB 30) human breast carcinoma cells.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
554300 Rev. 9
Antibody Details
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9G6

C-erbB-2 (also known as HER2/neu), a 185 kDa transmembrane glycoprotein, is a member of the type 1 growth factor receptor subfamily which also includes c-erbB-3, c-erbB-4 and the epidermal growth factor receptor (EGFR) (also known as c-erbB-1). Members of this receptor subfamily mediate the proliferation and differentiation of normal cells. They have a common structure consisting of an extracellular domain, a transmembrane region, and a cytoplasmic sequence. The extracellular regions contain two cysteine-rich domains, and the intracellular regions have sequence homology to known tyrosine kinases. C-erbB-3 reactivity has been detected in proximal kidney tubules, mucosal epithelium in the gastointenstinal tract, and squamous epithelium in skin. Most other normal adult tissue show little or no reactivity with antibodies against c-erbB-2, including breast, ovary, spleen, liver, bone marrow, prostate, adrenal, and lung. However, c-erbB-2 is overexpressed in many human breast, stomach, ovary and bladder carcinomas. EGFR, c-erbB-3 and c-erbB-4 are also overexpressed in various human tumor cells, and it is thought that aberrant activation of type 1 growth factor kinase activities may contribute to tumor progression. Clone 9G6 recognizes human c-erbB-2. Recombinant human c-erbB-2 was used as immunogen.

554300 Rev. 9
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
554300 Rev.9
Citations & References
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Development References (9)

  1. Carraway KL 3rd, Cantley LC. A neu acquaintance for erbB3 and erbB4: a role for receptor heterodimerization in growth signaling. Cell. 1994; 78(1):5-8. (Biology). View Reference
  2. Hancock MC, Langton BC, Chan T, et al. A monoclonal antibody against the c-erbB-2 protein enhances the cytotoxicity of cis-diamminedichloroplatinum against human breast and ovarian tumor cell lines. Cancer Res. 1991; 51(17):4575-4589. (Biology). View Reference
  3. In: Hesketh R. The Oncogene Handbook. New York: Academic Press; 1994:485-509.
  4. Penault-Llorca F, Adelaide J, Houvenaeghel G, Hassoun J, Birnbaum D, Jacquemier J. Optimization of immunohistochemical detection of ERBB2 in human breast cancer: impact of fixation. J Pathol. 1994; 173(1):65-75. (Clone-specific: Immunohistochemistry). View Reference
  5. Rubin SC, Finstad CL, Wong GY, Almadrones L, Plante M, Lloyd KO. Prognostic significance of HER-2/neu expression in advanced epithelial ovarian cancer: a multivariate analysis. Am J Obstet Gynecol. 1993; 168(1):162-169. (Clone-specific: Immunohistochemistry). View Reference
  6. Schwechheimer K, Laufle RM, Schmahl W, Knodlseder M, Fischer H, Hofler H. Expression of neu/c-erbB-2 in human brain tumors. Hum Pathol. 1994; 25(8):772-780. (Clone-specific: Immunohistochemistry). View Reference
  7. Singleton TP, Niehans GA, Gu F, et al. Detection of c-erbB-2 activation in paraffin-embedded tissue by immunohistochemistry. Hum Pathol. 1992; 23(10):1141-1150. (Clone-specific: Immunohistochemistry). View Reference
  8. Stål O, Sullivan S, Sun XF, Wingren S, Nordenskjöld B. Simultaneous analysis of c-erbB-2 expression and DNA content in breast cancer using flow cytometry. Cytometry. 1994; 16(2):160-168. (Clone-specific: Flow cytometry, Immunohistochemistry). View Reference
  9. van de Vijver MJ, Peterse JL, Mooi WJ, et al. Neu-protein overexpression in breast cancer. Association with comedo-type ductal carcinoma in situ and limited prognostic value in stage II breast cancer. N Engl J Med. 1988; 319(19):1239-1245. (Clone-specific: Immunohistochemistry, Immunoprecipitation). View Reference
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554300 Rev. 9

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.