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Flow cytometric analysis of osteocaclin expression in BGLAP-transfected HeLa cells. Untransfected (dashed line histogram) and human BGLAP-transfected (solid line histogram) HeLa cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No.558050). The cells were then washed and stained with PE Mouse Anti-Human Osteocalcin antibody (Cat. No. 564146) using BD Biosciences Protocol for Intracellular Immunofluorescent Staining. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Western blot analysis of osteocalcin expression by differentiated human Adipose Derived Stem Cells (ADSC). Cell lysates from undifferentiated (Left Blot) and differentiated (Right Blot) were electrophoresed (SDS-PAGE), transferred to membranes and then blotted using purified Mouse Anti-Osteocalcin antibody, Clone R14-707. Osteocaclin is identified as a band of ~11 kDa in the differentiated ADSC cell lysate when blotted with the R14-707 antibody at concentrations of 5 (lane 5), 2.5 (lane 6), 1.25 (lane 7), and 0.63 (lane 8) μg/mL.
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BD Pharmingen™ PE Mouse Anti-Human Osteocalcin

BD Pharmingen™ PE Mouse Anti-Human Osteocalcin
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Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The R14-707 monoclonal antibody specifically recognizes human Osteocalcin. Osteocalcin (OC/OCN/OSTCN) is encoded by the BGLAP gene and is also known as Bone gamma-carboxyglutamic acid-containing protein. Osteocalcin is an approximately ~11 kDa protein that is secreted by osteoblasts. Its carboxylated form binds hydroxyapatite and thus it is abundantly found in bone extracellular matrix. Its uncarboxylated form serves as a hormone that can positively regulate glucose homeostasis. Serum osteocalcin has also been used as a biomarker for bone formation and turnover.

Development References (5)
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Delmas PD, Stenner D, Wahner HW, Mann KG, Riggs BL. Increase in serum bone gamma-carboxyglutamic acid protein with aging in women: implications for the mechanism of age-related bone loss. J Clin Invest. 1983; 71(5):1316-1321. (Biology). View Reference
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Kapustin AN, Shanahan CM. Osteocalcin: A Novel Vascular Metabolic and Osteoinductive Factor. Arterioscler Thromb Vasc Biol. 2011; 31(10):2169-2171. (Biology). View Reference
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Levy RJ, Gundberg C, Scheinman R. The identification of the vitamin K-dependent bone protein osteocalcin as one of the gamma-carboxyglutamic acid containing proteins present in calcified atherosclerotic plaque and mineralized heart valves. Atherosclerosis. 1983; 46(1):49-56. (Biology). View Reference
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Rossini M, Gatti D, Viapiana O, et al. Short-term effects on bone turnover markers of a single high dose of oral vitamin D3. J Clin Endocrinol Metab. 2012; 97(4):E622-E626. (Biology). View Reference
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Schwetz V, Pieber T, Obermayer-Pietsch B. The endocrine role of the skeleton: background and clinical evidence. Eur J Endocrinol. 2012; 166(6):959-967. (Biology). View Reference
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