PE Mouse Anti-Human Integrin αvβ6

BD Pharmingen™ PE Mouse Anti-Human Integrin αvβ6

Name: PE Mouse Anti-Human Integrin αvβ6
Brand: BD Pharmingen™
SKU: 566922

Clone 10D5

(RUO)

Flow cytometric analysis of Integrin αvβ6 expression on human H441 cells. Cells from the human H441 (Papillary adenocarcinoma, ATCC HTB-174) cell line were stained with either PE Mouse IgG2a, κ Isotype Control (Cat No. 554648, dashed line histogram) or PE Mouse Anti-Human Integrin αvβ6 antibody (Cat No. 566922; solid line histogram) at 1 µg/test. The fluorescence histogram showing Integrin αvβ6 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable human H441 cells. Flow cytometry was performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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Product Details

Brand: BD Pharmingen™

Alternative Name: αVβ6; avb6; CD51/β6; ITGAV/ITGB6; Integrin alpha v beta 6

Reactivity: Human (QC Testing), Mouse (Reported)

Isotype: Mouse 129 x C57BL/6 IgG2a

Immunogen: 129/C57BL/6 β6 -/- Mice were mmunized with Human αvβ6 or Mouse Keratinocytes

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_2869950

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Recommended Assay Procedures

Note: The binding of the 10D5 antibody to αvβ6 is divalent-cation dependent. For optimal results, ensure ~ 1 mM Mg2+ is present in the staining and analysis buffers when using the 10D5 antibody for flow cytometric analysis. BD Pharmingen Stain Buffer (Cat. 554656/554657) contains Mg2+.

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Mouse IgG2a, κ Isotype Control RUO

Size 0.1 mg Cat No. 554648

566922 Rev. 1

Antibody Details

10D5

The 10D5 monoclonal antibody specifically recognizes Integrin alpha v beta 6 (αvβ6) that is also known as CD51/β6. This heterodimeric receptor is comprised of transmembrane type I alpha and beta chain glycoproteins that are encoded by ITGAV (integrin subunit alpha) and ITGB6 (integrin subunit beta 6), respectively. Like other integrins, αvβ6 serves as a transmembrane adhesion receptor that can tether the intracellular cytoskeleton to the extracellular matrix (ECM) by binding to R-G-D sequences of ECM protein ligands including fibronectin, vitronectin, and tenascin. Integrin αvβ6 likewise plays a key role in the activation of transforming growth factor beta-1 (TGFβ1) by its R-G-D-dependent release of TGFβ1 from regulatory Latency-associated peptide (LAP). Some viruses, such as. Coxsackieviruses and Herpes simplex virus-1 use Integrin αvβ6 as a receptor to infect cells. Integrin αvβ6 engages in bidirectional signaling, ie, outside-in and inside-out signaling, to regulate essential cellular functions. These include adhesion, migration, polarization, differentiation, and proliferation that are crucial to tissue homeostasis. Although not normally expressed on adult epithelial cells/keratinocytes, its expression is upregulated on these cells during the course of embryonic development, inflammation, tissue injury, wound healing, as well as malignant transformation. The 10D5 antibody blocks Integrin αvβ6-dependent cellular adhesion and migration and reportedly recognizes both human and mouse αvβ6 integrins. Binding of 10D5 antibody to αvβ6 is divalent-cation dependent.

566922 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

566922 Rev.1

Citations & References

Development References (14)

Allen MD, Thomas GJ, Clark S, et al. Altered microenvironment promotes progression of preinvasive breast cancer: myoepithelial expression of αvβ6 integrin in DCIS identifies high-risk patients and predicts recurrence.. Clin Cancer Res. 2014; 20(2):344-57. (Clone-specific: Blocking, Flow cytometry, Functional assay). View Reference
Bandyopadhyay A, Raghavan S. Defining the role of integrin alphavbeta6 in cancer.. Curr Drug Targets. 2009; 10(7):645-52. (Biology). View Reference
Bates RC, Bellovin DI, Brown C, et al. Transcriptional activation of integrin beta6 during the epithelial-mesenchymal transition defines a novel prognostic indicator of aggressive colon carcinoma. J Clin Invest. 2005; 115(2):339-47. (Clone-specific: Blocking). View Reference
Huang X, Wu J, Spong S, Sheppard D. The integrin alphavbeta6 is critical for keratinocyte migration on both its known ligand, fibronectin, and on vitronectin. J Cell Sci. 1998; 111(15):2189-95. (Immunogen: Flow cytometry, Functional assay, Immunoprecipitation, Inhibition). View Reference
Jackson T, Sheppard D, Denyer M, Blakemore W, King AM. The epithelial integrin αvβ6 is a receptor for foot-and-mouth disease virus. J Virol. 2000; 74(11):4949-56. (Clone-specific: Blocking). View Reference
Jovanovic J, Takagi J, Choulier L, et al. alphaVbeta6 is a novel receptor for human fibrillin-1. Comparative studies of molecular determinants underlying integrin-rgd affinity and specificity.. J Biol Chem. 2007; 282(9):6743-51. (Clone-specific: Blocking, Flow cytometry). View Reference
Koivisto L, Bi J, Häkkinen L, Larjava H. Integrin αvβ6: Structure, function and role in health and disease.. Int J Biochem Cell Biol. 2018; 99:186-196. (Biology). View Reference
Li X, Yang Y, Hu Y, et al. Alphavbeta6-Fyn signaling promotes oral cancer progression.. J Biol Chem. 2003; 278(43):41646-53. (Clone-specific: Blocking). View Reference
Maier S, Paulsson M, Hartmann U. The widely expressed extracellular matrix protein SMOC-2 promotes keratinocyte attachment and migration.. Exp Cell Res. 2008; 314(13):2477-87. (Clone-specific: Blocking). View Reference
Miller LC, Blakemore W, Sheppard D, Atakilit A, King AM, Jackson T. Role of the cytoplasmic domain of the beta-subunit of integrin alpha(v)beta6 in infection by foot-and-mouth disease virus. J Virol. 2001; 75(9):4158-64. (Clone-specific: Blocking). View Reference
Munger JS, Huang X, Kawakatsu H, et al. The integrin alpha v beta 6 binds and activates latent TGF beta 1: a mechanism for regulating pulmonary inflammation and fibrosis.. Cell. 1999; 96(3):319-28. (Clone-specific: Blocking, Functional assay). View Reference
Thomas GJ, Lewis MP, Whawell SA, et al. Expression of the alphavbeta6 integrin promotes migration and invasion in squamous carcinoma cells.. J Invest Dermatol. 2001; 117(1):67-73. (Clone-specific: Blocking). View Reference
Thomas GJ, Nyström ML, Marshall JF. Alphavbeta6 integrin in wound healing and cancer of the oral cavity.. J Oral Pathol Med. 2006; 35(1):1-10. (Biology). View Reference
Williams CH, Kajander T, Hyypiä T, Jackson T, Sheppard D, Stanway G. Integrin alpha v beta 6 is an RGD-dependent receptor for coxsackievirus A9. J Virol. 2004; 78(13):6967-73. (Clone-specific). View Reference

View All (14)

566922 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.