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PE Mouse Anti-Human Delta-Like Protein 4
PE Mouse Anti-Human Delta-Like Protein 4

Flow cytometric analysis of human Delta-Like Protein 4 on DLL4-transfected CHO cells. Human DLL4-transfected Chinese Hamster Ovary (CHO) cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dotted line histogram) or PE Mouse Anti-Human Delta-Like Protein 4 antibody (Cat. No. 564412; solid line histogram). Fluorescence histograms showing the expression of Delta-Like Protein 4 (or Ig Isotype Control staining) were generated for gated events with the forward and side light-scatter characteristics of viable CHO cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Flow cytometric analysis of human Delta-Like Protein 4 on DLL4-transfected CHO cells. Human DLL4-transfected Chinese Hamster Ovary (CHO) cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dotted line histogram) or PE Mouse Anti-Human Delta-Like Protein 4 antibody (Cat. No. 564412; solid line histogram). Fluorescence histograms showing the expression of Delta-Like Protein 4 (or Ig Isotype Control staining) were generated for gated events with the forward and side light-scatter characteristics of viable CHO cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Product Details
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BD Pharmingen™
DLL4; Delta-like 4; DL4; Delta ligand 4; Delta4; Notch ligand DLL4
Human (QC Testing)
Mouse IgG1, κ
Human Delta-Like Protein 4 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738798
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
564412 Rev. 1
Antibody Details
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MHD4-46

The MHD4-46 monoclonal antibody specifically binds to Delta-Like Protein 4 which is also known as Delta4, DLL4, and DL4. Delta-Like Protein 4 is a type 1 transmembrane glycoprotein which belongs to the Delta/Serrate/Jagged Family of Notch receptor ligands. The Notch family of transmembrane receptors and their ligands play key roles in various developmental and postnatal processes by controlling cell-fate decisions. Delta-Like Protein 4 is expressed by vascular endothelial cells within certain normal tissues and tumors. It signals through Notch1 and Notch 4 Receptors and plays a critical role in vascular development and homeostasis. Delta-Like Protein 4 expression is upregulated in response to vascular endothelial growth factor (VEGF) and regulates endothelial cell proliferation during vascular sprouting. Delta-Like Protein 4 is also expressed by activated human monocytes and dendritic cells that have been stimulated with proinflammatory activators such as lipopolysaccharide. Recent evidence suggests that Delta-Like Protein 4 may contribute to the T cell potential of CD34+ cells and to the growth of various tumors, and promote tumor cell infiltration.

During product development, the specificity of the purified MHD4-46 antibody (Cat. No. 564589) was confirmed by immunohistochemical staining of  acetone-fixed frozen sections of human placenta. Vascular endothelial cells were specifically stained (data not shown).

564412 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
564412 Rev.1
Citations & References
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Development References (10)

  1. Artavanis-Tsakonas S, Rand MD, Lake RJ. Notch signaling: cell fate control and signal integration in development.. Science. 1999; 284(5415):770-6. (Biology). View Reference
  2. Fung E, Tang SM, Canner JP, et al. Delta-like 4 induces notch signaling in macrophages: implications for inflammation. Circulation. 207; 115(23):2948-2956. (Biology). View Reference
  3. Gurney A, Hoey T. Anti-DLL4, a cancer therapeutic with multiple mechanisms of action. Vasc Cell. 2011; 3(18):21. (Biology). View Reference
  4. Herr F, Schreiner I, Baal N, Pfarrer C, Zygmunt M. Expression patterns of Notch receptors and their ligands Jagged and Delta in human placenta. Placenta. 2011; 32(8):554-563. (Biology). View Reference
  5. Lefort N, Benne C, Lelievre JD, et al. Short exposure to Notch ligand Delta-4 is sufficient to induce T-cell differentiation program and to increase the T cell potential of primary human CD34+ cells. Exp Hematol. 2006; 34(12):1720-1729. (Biology). View Reference
  6. Mailhos C, Modlich U, Lewis J, Harris A, Bicknell R, Ish-Horowicz D. Delta4, an endothelial specific notch ligand expressed at sites of physiological and tumor angiogenesis. Differentiation. 2001; 69(2-3):135-144. (Biology). View Reference
  7. Sekine C, Koyanagi A, Koyama N, Hozumi K, Chiba S, Yagita H. Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes. Arthritis Res Ther. 2012; 14(2):R45-R58. (Immunogen: Flow cytometry). View Reference
  8. Shutter JR, Scully S, Fan W, et al. Dll4, a novel Notch ligand expressed in arterial endothelium. Genes Dev. 2000; 14:1313-1318. (Biology). View Reference
  9. Shutter JR, Scully S, Fan W, et al. Molecular cloning of delta-4, a new mouse and human Notch ligand. J Biochem (Tokyo). 2001; 129(1):27-34. (Biology). View Reference
  10. Wakui M, Nakano K, Matsushita S. Notch ligand mRNA levels of human APCs predict Th1/Th2-promoting activities. Biochem Biophys Res Commun. 2007; 358(2):596-601. (Biology). View Reference
View All (10) View Less
564412 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.