Flow cytometric analysis of c-MAF expression in human and mouse leucocytes. Human peripheral blood mononuclear cells (PBMC; Left Panel) and C57BL/6 mouse peritoneal exudate cells (PEC; Right Panel) were preincubated with either BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/564220), or Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142), respectively. The cells were then bulk treated with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and washed with TF Perm/Wash. The human PBMC were then stained with Alexa Fluor® 700 Mouse anti-Human CD11b antibody (Cat. No. 557918), and either PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648; Left Plot), or PE Mouse Anti-c-Maf antibody (Cat. No. 565795; Right Plot). The mouse PEC were stained with BD Horizon™ V500 Rat anti-Mouse CD11b antibody, and either PE Mouse IgG2a, κ Isotype Control (Left Plot), or PE Mouse Anti-c-Maf antibody (Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD11b versus c-MAF (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact human PBMC or mouse PEC, respectively. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
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Flow cytometric analysis of c-MAF expression in human and mouse leucocytes. Human peripheral blood mononuclear cells (PBMC; Left Panel) and C57BL/6 mouse peritoneal exudate cells (PEC; Right Panel) were preincubated with either BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/564220), or Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142), respectively. The cells were then bulk treated with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and washed with TF Perm/Wash. The human PBMC were then stained with Alexa Fluor® 700 Mouse anti-Human CD11b antibody (Cat. No. 557918), and either PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648; Left Plot), or PE Mouse Anti-c-Maf antibody (Cat. No. 565795; Right Plot). The mouse PEC were stained with BD Horizon™ V500 Rat anti-Mouse CD11b antibody, and either PE Mouse IgG2a, κ Isotype Control (Left Plot), or PE Mouse Anti-c-Maf antibody (Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD11b versus c-MAF (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact human PBMC or mouse PEC, respectively. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
MAF; v-MAF; MAF; AYGRP; CCA4; CTRCT21
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG2a, κ
Human c-MAF Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739359
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.1 mg
Cat No.
553141
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.5 mg
Cat No.
553142
Human BD Fc Block™ RUO
Size
50 µg
Cat No.
564219
Human BD Fc Block™ RUO
Size
0.25 mg
Cat No.
564220
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
565795 Rev. 1
Antibody Details
T54-853
The T54-853 monoclonal antibody recognizes c-Maf, a transcription factor that is also known as, MAF, c-Maf protooncogene, or V-maf musculoaponeurotic fibrosarcoma oncogene homolog. c-Maf belongs to the basic leucine zipper (bZIP) family of transcription factors. c-Maf acts as a homodimer or can dimerize with other bZIP transcription factors to either activate or repress the expression of various genes. It plays multiple roles in the differentiation and effector functions of leucocytes, including macrophages, Th2 cells, Th17 cells, and T follicular helper (Tfh) cells. c-Maf drives the expression of IL-10 by activated macrophages, IL-21 by activated Tfh and Th17 cells, and IL-4 by Th2 cells.
565795 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565795 Rev.1
Citations & References
View product citations for antibody "565795" on CiteAb
Development References (8)
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Apetoh L, Quintana FJ, Pot C, et al. The aryl hydrocarbon receptor interacts with c-Maf to promote the differentiation of type 1 regulatory T cells induced by IL-27.. Nat Immunol. 2010; 11(9):854-61. (Biology). View Reference
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Barros MH, Hauck F, Dreyer JH, Kempkes B, Niedobitek G. Macrophage polarisation: an immunohistochemical approach for identifying M1 and M2 macrophages.. PLoS ONE. 2013; 8(11):e80908. (Biology). View Reference
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Dhiman R, Bandaru A, Barnes PF, et al. c-Maf-dependent growth of Mycobacterium tuberculosis in a CD14(hi) subpopulation of monocyte-derived macrophages.. J Immunol. 2011; 186(3):1638-45. (Biology). View Reference
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Grigoryan G, Reinke AW, Keating AE. Design of protein-interaction specificity gives selective bZIP-binding peptides.. Nature. 2009; 458(7240):859-64. (Biology). View Reference
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Hiramatsu Y, Suto A, Kashiwakuma D, et al. c-Maf activates the promoter and enhancer of the IL-21 gene, and TGF-beta inhibits c-Maf-induced IL-21 production in CD4+ T cells.. J Leukoc Biol. 2010; 87(4):703-12. (Biology). View Reference
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Ho IC, Hodge MR, Rooney JW, Glimcher LH. The proto-oncogene c-maf is responsible for tissue-specific expression of interleukin-4.. Cell. 1996; 85(7):973-83. (Biology). View Reference
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Kroenke MA, Eto D, Locci M, et al. Bcl6 and Maf cooperate to instruct human follicular helper CD4 T cell differentiation.. J Immunol. 2012; 188(8):3734-44. (Biology). View Reference
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van den Bosch MW, Palsson-Mcdermott E, Johnson DS, O'Neill LA. LPS induces the degradation of programmed cell death protein 4 (PDCD4) to release Twist2, activating c-Maf transcription to promote interleukin-10 production.. J Biol Chem. 2014; 289(33):22980-90. (Biology). View Reference
565795 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.