Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- BD Horizon Brilliant Violet 786 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
- Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
Companion Products
The YN1/1.7.4 monoclonal antibody specifically binds to CD54 which is also known as Intercellular adhesion molecule 1 (ICAM-1). CD54 (ICAM-1) is a ~90-110 kDa single-pass type I transmembrane glycoprotein that is encoded by Icam1 which belongs to the Immunoglobulin gene superfamily (IgSF). This adhesion molecule is comprised of five IgC2-like domains in its extracellular region, a transmembrane segment, and a cytoplasmic tail. It is expressed on T and B lymphocytes, monocytes, macrophages, dendritic cells (DC), endothelial cells, and epithelial cells. Through binding to cell surface ligands including LFA1 (CD11a/CD18) and Mac-1 (CD11b/CD18), CD54 (ICAM-1) serves to mediate intercellular connections between T cells, B cells, endothelial cells, antigen-presenting cells, and target cells. Its expression is upregulated upon stimulation by inflammatory mediators including proinflammatory cytokines such as IL-1, IFN-γ, and TNF as well as lipopolysaccharide (LPS). CD54 (ICAM-1) participates in leucocyte trans-endothelial migration and trafficking, inflammatory reactions, and antigen-specific immune responses. The YN1/1.7.4 antibody reportedly blocks in vitro and in vivo intercellular adhesion functions mediated by CD54 (ICAM-1) involved in inflammation and immune responses.
Development References (3)
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Horley KJ, Carpenito C, Baker B, Takei F. Molecular cloning of murine intercellular adhesion molecule (ICAM-1).. EMBO J. 1989; 8(10):2889-96. (Clone-specific). View Reference
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Takei F. Inhibition of mixed lymphocyte response by a rat monoclonal antibody to a novel murine lymphocyte activation antigen (MALA-2).. J Immunol. 1985; 134(3):1403-7. (Immunogen: Activation, Flow cytometry, Functional assay, Immunoprecipitation, Inhibition, Radioimmunoassay, Stimulation). View Reference
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Wang Q, Zhang M, Ding G, et al. Anti-ICAM-1 antibody and CTLA-4Ig synergistically enhance immature dendritic cells to induce donor-specific immune tolerance in vivo.. Immunol Lett. 2003; 90(1):33-42. (Clone-specific). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.