The HIP8 monoclonal antibody specifically binds to the α-chain of CD41. CD41 is also known as Integrin αIIb or Platelet GPIIb. The calcium-dependent complex of CD41 and CD61 (β3 integrin or GPIIIa) is normally expressed on platelets and megakaryocytes. The CD41/CD61 complex is the receptor for fibrinogen, fibronectin and von Willebrand factor, and mediates platelet adhesion and aggregation. CD41 (clone HIP8) completely inhibits ADP-, epinephrine-, and collagen-induced platelet activation, and partially inhibits ristocetin- and thrombin-induced platelet activation. This antibody is useful in the morphological and physiological studies of platelets and megakaryocytes.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor® 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.