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BV421 Mouse Anti-Human CD26
BV421 Mouse Anti-Human CD26

Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human CD26 antibody (Cat. No. 744769)  on human peripheral blood.  Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.

Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human CD26 antibody (Cat. No. 744769)  on human peripheral blood.  Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.

Product Details
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BD OptiBuild™
DPP IV; DPPIV; DPP4; Dipeptidyl peptidase IV; ADABP; ADCP-2; TP103
Human (Tested in Development)
Mouse BALB/c IgG2a, κ
E1αPGF/JY cells
Flow cytometry (Qualified)
0.2 mg/ml
1803
AB_2742467
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
744769 Rev. 2
Antibody Details
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L272

The L272 monoclonal antibody specifically recognizes CD26 which is also known as Dipeptidyl peptidase IV (DPP IV), a serine protease. CD26 is a ~120 kDa type II transmembrane glycoprotein that is encoded by DPP4 and belongs to the DPP4 activity and/or structure homologue (DASH) protein family. It is associated with the binding of the TAT transactivating protein of the HIV. CD26 and CD45 act in a costimulatory fashion on T lymphocytes. Present on peripheral blood T lymphocytes, the CD26 antigen is upregulated on phytohemagglutinin (PHA) and concanavalin A (Con A)-stimulated peripheral blood mononuclear cells (PBMCs). The CD26 antigen is found on approximately 50% of CD4 and approximately 30% of CD8 cells. It is also found on some mature thymocytes, B cells, natural killer (NK) cells, monocytes, macrophages, epithelial cells, EBV transformed B-cell lines, and hairy cell leukemia. Absolute numbers of CD4+CD26+ and CD8+CD26+ cells are reported to be lower in HIV-positive individuals. The CD26-CD4+ cell appears to be a reservoir for HIV. CD26 bright CD4 lymphocytes are CD25+ and CD45RO+ memory T lymphocytes. The CD26 antigen can serve as a receptor for the coronavirus, MERS-CoV.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

744769 Rev. 2
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
744769 Rev.2
Citations & References
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Development References (11)

  1. Bleul CC, Wu L, Hoxie JA, Springer TA, Mackay CR. The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.. Proc Natl Acad Sci U S A. 1997; 94(5):1925-1930. (Clone-specific: Flow cytometry). View Reference
  2. Gutheil WG, Subramanyam M, Flentke GR, et al. Human immunodeficiency virus 1 Tat binds to dipeptidyl aminopeptidase IV (CD26): a possible mechanism for Tat's immunosuppressive activity.. Proc Natl Acad Sci USA. 1994; 91(14):6594-8. (Biology). View Reference
  3. Lu G, Hu Y, Wang Q, et al. Molecular basis of binding between novel human coronavirus MERS-CoV and its receptor CD26.. Nature. 2013; 500(7461):227-31. (Biology). View Reference
  4. Morimoto C, Kameoka J, Tanaka T, Schlossman S. Overview of CD26. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1105-1114.
  5. Muñoz E, Blazquez MV, Madueño JA, Rubio G, Peña J. CD26 induces T-cell proliferation by tyrosine protein phosphorylation.. Immunology. 1992; 77(1):43-50. (Biology). View Reference
  6. Plana M, Font J, Viñas O, Martorell J, Ingelmo M, Vives J. Responsiveness of T lymphocytes from systemic lupus erythematosus to signals provided through CD26 antigen.. Clin Immunol Immunopathol. 1994; 72(2):227-32. (Biology). View Reference
  7. Stein H, Schwarting R, Niedobitek G. Cluster report: CD26. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:412.
  8. Ulmer AJ, Mattern T, Flad HD. Expression of CD26 (dipeptidyl peptidase IV) on memory and naive T lymphocytes.. Scand J Immunol. 1992; 35(5):551-9. (Biology). View Reference
  9. Vanham G, Kestens L, De Meester I, et al. Decreased expression of the memory marker CD26 on both CD4+ and CD8+ T lymphocytes of HIV-infected subjects.. J Acquir Immune Defic Syndr. 1993; 6(7):749-57. (Biology). View Reference
  10. Zola H. CD26. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:81-82.
  11. van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Clone-specific: Flow cytometry). View Reference
View All (11) View Less
744769 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.