The CK41 monoclonal antibody specifically binds to Nectin-1, which is also known as, CD111, Poliovirus receptor-related protein 1(PRR1, PVRL1), Herpes virus entry mediator C (HveC), or Herpesvirus Ig-like receptor (HIgR). Nectin-1 is a type I transmembrane glycoprotein that belongs to the Ig gene superfamily. CD111 is expressed on a variety of cell types including, epithelial cells, endothelial cells, neuronal cells, megakaryocytes, and CD34 positive stem cells. CD111 functions as a receptor for herpes simplex virus 1 (HSV 1) and HSV 2. Nectin-1 also serves as an intercellular adhesion molecule helping to form junctions between cells, eg, between endothelial cells or epithelial cells. Nectin-1 can bind to other Nectin-1 molecules and Nectin family members and to CD155 (Poliovirus Receptor/PVR).
The antibody was conjugated to BD Horizon™ BUV661 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 661-nm. BD Horizon Brilliant BUV661 can be excited by the ultraviolet laser (355 nm) and detected with a 670/25 filter and a 630 nm LP. Due to cross laser excitation of this dye, there may be significant spillover into channels detecting APC-like emissions (eg, 670/25-nm filter).
Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV661 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV661 reagents (eg, CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone-specific compensation controls when using these reagents.