The 10/78 monoclonal antibody recognizes the rat CD161 proteins, CD161a (also known as, Klrb1a, or Nkrp1a/NKR-P1A), and CD161b (Klrb1b, Nkrp1b/NKR-P1B). These type II transmembrane glycoproteins have an extracellular C-type lectin domain and thus belong to the C-type lectin superfamily. These CD161 proteins form ~ 60 kDa homodimers that are expressed on natural killer cells and subsets of T lymphocytes, activated monocytes, and dendritic cells. The 10/78 antibody competes with the previously-described 3.2.3 antibody for binding to these CD161 proteins. CD161 molecules are C-type lectin-like receptors that can either activate (CD161a) or inhibit (CD161b) effector leucocyte responses, eg, cytotoxicity or cytokine production, against target cells which express C-type lectin-like related (Clr) molecules. Although several members of the Klrb1 gene family have been identified in the mouse and rat, only a single human KLRB1 homolog has been discovered.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).