The MMRI-7 monoclonal antibody specifically recognizes CD205 which is also known as DEC-205, Lymphocyte antigen 75 (Ly-75), or C-type lectin domain family 13 member B (CLEC13B). CD205 is a ~205 kDa type I transmembrane glycoprotein with C-type lectin external domains. DEC-205 is expressed on myeloid and plasmacytoid dendritic cells, thymic epithelial cells, and at lower levels on monocytes, T cells, B cells, and natural killer (NK) cells. DEC-205 likely functions as an endocytic receptor and is involved in antigen uptake and processing.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).