The 4A6 monoclonal antibody specifically recognizes mouse PDC-TREM, also known as TREM-4, which is a member of the Triggering Receptor Expressed on Myeloid cells (TREM) family of protein-binding receptors that regulate innate immune responses. Stimulation of plasmacytoid dendritic cells (pDCs) through toll-like receptors (TLRs) induces type I interferon production and PDC-TREM expression. Cell-surface expression and immune regulatory signaling by PDC-TREM require its association with Plexin-A1 and DNAX-activation Protein 12 (DAP12) and further augments type I interferon production by pDCs.
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.