The V65 monoclonal antibody specifically recognizes the γδ T-cell Receptor (TCR) found on CD3+ αβ TCR-peripheral T lymphocytes, on a small subset of intestinal intraepithelial lymphocytes, and on dendritic epidermal T cells. Cross-linked V65 mAb induces cytokine-dependent T-cell mitogenesis in vitro, and in vivo treatment can significantly deplete peripheral γδ TCR-expressing T cells. A suspension of rat CD3+ αβ TCR-T-cell hybridoma III.89.1.4 was used as the source of the immunogen.
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.