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Biotin Mouse Anti-Rat and Anti-Hamster Ig, κ Light Chain
Product Details
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BD Pharmingen™
Rat (QC Testing), Armenian Hamster, Syrian Hamster (Tested in Development)
Mouse SJL IgG2a, κ
Pooled Rat IgG
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
500 µg/ml
AB_393616
Aqueous buffered solution containing BSA, protein stabilizer, goat serum, and ≤ 0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550336 Rev. 5
Antibody Details
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RG7/7.6

The RG7/7.6 antibody reacts strongly with rat Igs bearing - light chain of the Igk-1[a]allotype (e.g., AO, AUG, BN, BUF, LEW, LOU, PVG, WAG, WF), weakly with Igk-1[b] light chains (e.g., ACI, COP, DA, F344, SHR), and it does not react with λ light chain nor heavy chains. At the time that this antibody was characterized, the Igk-1[a] allotype was designated - 1b, and Igk-1[b] was -1a; the current nomenclature was standardized at the Fourth International Workshop on Allogenic Systems in the Rat. The RG7/7.6 antibody also strongly cross-reacts with Syrian and Armenian hamster Ig - light chain.

550336 Rev. 5
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
550336 Rev.5
Citations & References
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Development References (2)

  1. Butcher GW. A list of monoclonal antibodies specific for alloantigens of the rat. J Immunogenet. 1987; 14(2-3):163-176. (Clone-specific). View Reference
  2. Springer TA, Bhattacharya A, Cardoza JT, Sanchez-Madrid F. Monoclonal antibodies specific for rat IgG1, IgG2a, and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies. Hybridoma. 1982; 1(3):257-273. (Clone-specific). View Reference
550336 Rev. 5

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.