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Biotin Mouse Anti-Mouse IgD[b]
Product Details
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BD Pharmingen™
Igh-5b; Ighd; immunoglobulin heavy constant delta
Mouse (QC Testing)
Mouse BALB/c IgG1, κ
Not reported
Flow cytometry (Routinely Tested)
0.5 mg/ml
380797
AB_394892
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553509 Rev. 11
Antibody Details
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217-170

The 217-170 monoclonal antibody specifically recognizes mouse IgD of Igh-C[b] haplotype (eg, C57BL/6, C57BL/10, SJL). It does not react with IgD of Igh-C[a] or related haplotypes (eg, BALB/c, AKR, CBA, C3H/He, C58, DBA/1, DBA/2, NZB, PL, RIII), or Igh-C[e] haplotype (eg, A).  217-170 antibody is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis.  It has not been shown to stimulate B-cell proliferation.

553509 Rev. 11
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
553509 Rev.11

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.