Alexa Fluor® 647 Mouse Anti-Human LAG-3 (CD223)
Multicolor flow cytometric analysis of LAG-3 (CD223) expression on unstimulated (Top Plots) and stimulated (Bottom Plots) human peripheral blood lymphocytes. Human peripheral blood mononuclear cells (PBMC) were cultured for 3 days with plate-bound Anti-Human CD3 (Cat. No. 555329; 10 µg/mL for coating) and soluble Anti-Human CD28 (Cat. No. 555725; 1 µg/mL) antibodies, and Human Recombinant IL-2 (Cat. No. 554603; 10 ng/mL). Unstimulated PBMC (from the same donor) and the stimulated PBMC were stained with PE Mouse Anti-Human CD8 (Cat. No. 555367/557086/561949/561950), BD Horizon™ BV421 Mouse Anti-Human CD279 (PD-1) (Cat. No. 562516/565935), and Alexa Fluor® 647 Mouse Anti-Human LAG-3 (CD223) (Cat. No. 565716/565717) antibodies. Two-color flow cytometric contour plots showing the correlated expression of LAG-3 (CD223) versus CD8 (Left Plots), or LAG-3 (CD223) versus CD279 (PD-1) (Right Plots) were derived from gated events with the forward and side light-scatter characteristics of viable unstimulated (Top Plots) or stimulated (Bottom Plots) lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human LAG-3 (CD223)
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