Multiparameter flow cytometric analysis of IL-10Rβ (CD210b) expression on human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human HLA-DR (Cat. No. 555812/556644/560943) and FITC Mouse Anti-Human CD14 (Cat. No. 555397/557153/561712) antibodies and either Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No. 557714) or Alexa Fluor® 647 Mouse Anti-Human IL-10Rβ (CD210b) antibody (Cat. No. 564372). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Panel 1. Two-parameter contour plots show the correlated expression of isotype control (Left Plot) or IL-10Rβ (CD210b) (Right Plot) versus side-light scatter (SSC-A) characteristics for gated events with the forward light scatter properties of total viable leucocytes. Panel 2. For gated events with the forward and side-light scatter characteristics of viable mononuclear leucocytes, the two-color contour plots show the correlated expression of CD14 (Left Plot) or IL-10Rβ (CD210b) (Right Plot) versus HLA-DR for HLA-DR+ (and HLA-DR-) lymphocytes and CD14+HLA-DR+ monocytes.
Multiparameter flow cytometric analysis of IL-10Rβ (CD210b) expression on human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human HLA-DR (Cat. No. 555812/556644/560943) and FITC Mouse Anti-Human CD14 (Cat. No. 555397/557153/561712) antibodies and either Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No. 557714) or Alexa Fluor® 647 Mouse Anti-Human IL-10Rβ (CD210b) antibody (Cat. No. 564372). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Panel 1. Two-parameter contour plots show the correlated expression of isotype control (Left Plot) or IL-10Rβ (CD210b) (Right Plot) versus side-light scatter (SSC-A) characteristics for gated events with the forward light scatter properties of total viable leucocytes. Panel 2. For gated events with the forward and side-light scatter characteristics of viable mononuclear leucocytes, the two-color contour plots show the correlated expression of CD14 (Left Plot) or IL-10Rβ (CD210b) (Right Plot) versus HLA-DR for HLA-DR+ (and HLA-DR-) lymphocytes and CD14+HLA-DR+ monocytes.
Product Details
BD Pharmingen™
IL-10R2; IL-10RB; IL-10Rβ; IL10RB; IL-10 Receptor beta; CRFB4
Human (QC Testing)
Mouse IgG1, κ
Human IL-10 Rβ Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl/test
AB_2738774
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Alexa Fluor® 647 Mouse IgG1 κ Isotype Control RUO
Size
100 Tests
Cat No.
557714
PE Mouse Anti-Human HLA-DR RUO
Size
100 Tests
Cat No.
555812
PE Mouse Anti-Human HLA-DR RUO
Size
50 Tests
Cat No.
556644
PE Mouse Anti-Human HLA-DR RUO
Size
25 Tests
Cat No.
560943
564372 Rev. 2
Antibody Details
90220
The 90220 monoclonal antibody specifically binds to the beta subunit (IL-10R beta/IL-10RB/IL-10Rβ) of the heteromeric IL-10 Receptor complex. This type I transmembrane glycoprotein is also known as, subunit 2 (IL-10R2) or CD210b. The IL-10 Receptor complex is formed by the binding of dimeric IL-10 to two cell surface alpha subunits of the IL-10 Receptor (CD210a/IL-10RA/IL-10Rα). This complex can then recruit two CD210b subunits resulting in the transduction of intracellular JAK/STAT pathway signals. CD210b is also a component of cytokine receptor complexes specific for IL-22, IL-26, IL-28 and IL-29. CD210b is expressed by a variety of cell types including T cells, B cells, NK cells, monocytes and macrophages.
564372 Rev. 2
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
564372 Rev.2
Citations & References
Development References (5)
-
Crepaldi L, Gasperini S, Lapinet JA, et al. Up-regulation of IL-10R1 expression is required to render human neutrophils fully responsive to IL-10.. J Immunol. 2001; 167(4):2312-22. (Biology). View Reference
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Donnelly RP, Sheikh F, Kotenko SV, Dickensheets H. The expanded family of class II cytokines that share the IL-10 receptor-2 (IL-10R2) chain. J Leukoc Biol. 2004; 76(2):314-321. (Biology). View Reference
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Koppelman B, Neefjes JJ, de Vries JE, de Waal Malefyt R. Interleukin-10 down-regulates MHC class II alphabeta peptide complexes at the plasma membrane of monocytes by affecting arrival and recycling. Immunity. 1997; (6):861-871. (Biology). View Reference
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Kotenko SV, Krause CD, Izotova LS, Pollack BP, Wu W, Pestka S. Identification and functional characterization of a second chain of the interleukin-10 receptor complex. EMBO J. 1997; 16(19):5894-5903. (Biology). View Reference
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Liu Y, Wei SH, Ho AS, de Waal Malefyt R, Moore KW. Expression cloning and characterization of a human IL-10 receptor. J Immunol. 1995; 152(4):1821-1829. (Biology). View Reference
564372 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
