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Rat Compensation Set

Rat Compensation Set

(RUO)
Product Details
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BD Pharmingen™
Flow cytometry (Routinely Tested)
RUO
AB_2869212


Description

The Rat Compensation Set is intended to be used with the Rat T lymphocyte Cocktail (Cat. no. 558493) or the Rat T/B/NK Cell Cocktail (Cat. no. 558495) for instrument compensation.  Compensation is the process by which the effect of spectral overlap among flurochromes can be corrected minimizing fluorescence signal spillover in neighboring channels.  Traditionally, compensation values are entered into software programs allowing for the adjustment among the overlapping flurochrome emissions.  These values can be achieved using single color controls, and verified using fluorescence-minus-one controls, or ad-mixtures of single color controls.  Use this set to properly adjust your FITC/PE/APC compensation matrix for the Rat T Lymphocyte or Rat T/B/NK Cell Cocktails.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
558517 Rev. 1
Components
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Description Quantity/Size Part Number EntrezGene ID
APC Mouse anti-Rat CD3 20 Tests (1 ea) 51-9004354 N/A
PE Mouse anti-Rat CD4 20 Tests (1 ea) 51-9004355 N/A
FITC Mouse anti-Rat CD8a 20 Tests (1 ea) 51-9004686 N/A
558517 Rev. 1
Citations & References
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Development References (1)

  1. Shapiro HM. Practical flow cytometry, 3rd ed.. New York: Wiley-Liss; 1995:18-19.
558517 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.