The Rat Compensation Set is intended to be used with the Rat T lymphocyte Cocktail (Cat. no. 558493) or the Rat T/B/NK Cell Cocktail (Cat. no. 558495) for instrument compensation. Compensation is the process by which the effect of spectral overlap among flurochromes can be corrected minimizing fluorescence signal spillover in neighboring channels. Traditionally, compensation values are entered into software programs allowing for the adjustment among the overlapping flurochrome emissions. These values can be achieved using single color controls, and verified using fluorescence-minus-one controls, or ad-mixtures of single color controls. Use this set to properly adjust your FITC/PE/APC compensation matrix for the Rat T Lymphocyte or Rat T/B/NK Cell Cocktails.