p53 is a 53 kDa nuclear phosphoprotein that acts as a tumor suppressor protein, and is involved in inhibiting cell proliferation when DNA damage occurs. The gene for p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpresssed in a variety of transformed cells and wildtype p53 forms specific complexes with several viral oncogenes including SV40 large T, E1b from adenovirus, and E6 from human papilloma virus. Wildtype p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle.
Clone G59-12 recognizes mutant and wild type human, mouse, and rat p53 tumor suppressor protein. Recombinant full-length human p53 was used as immunogen. The G59-12 clone was originally characterized by western blot analysis, immunoprecipitation and immunohistochemical staining. MOPC-21 antibody has unknown specificity. The G59-12 and MOPC-21 FITC conjugates are matched in F/P ratios determined experimentally by flow cytometric analysis.