Cyclins and cyclin-dependent kinases (cdks) are subunits of cell cycle dependent protein kinases that regulate key events during the progression of the cell cycle. Specific substrates for cdk/cyclin kinases include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large-T), tumor suppressor genes (the retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. Human cyclin D1 migrates at a molecular weight of 36 kDa in SDS/PAGE.
Clone G124-326 recognizes human Cyclin D1. It does not cross-react with human cyclins D2 and D3. Recombinant full-length human Cyclin D1 was used as immunogen. The G124-326 antibody was initially characterized by immunoprecipitation and western blot analysis. In publications it has been used for western blot analysis, flow cytometric analysis, and immunohistochemistry of frozen tissue sections.
Clone MOPC-21 is a mouse IgG1 isotype (negative) control. The MOPC-21 antibody has unknown specificity. The G124-326 and MOPC-21 FITC conjugates are matched in F/P ratios. The optimum F/P ratio was experimentally determined by flow cytometric analysis.