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Purified Mouse IgA, κ Isotype Control
Product Details
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BD Pharmingen™
Mouse BALB/c IgA, κ
ELISA Standard (Routinely Tested), Flow cytometry, Isotype control (Tested During Development), Immunohistochemistry, Intracellular staining (flow cytometry) (Not Recommended)
0.5 mg/ml
AB_479590
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

The M18-254 clone is useful as a standard in sandwich ELISA, utilizing Cat. No. 556969 as the capture antibody and Cat. No. 556978 as the biotinylated detection antibody.  It is useful as an isotype-matched negative control for immunostaining of unfixed, live cell suspensions. This product is not recommended for use as an isotype control for intracellular or immunohistochemical staining applications.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553476 Rev. 12
Antibody Details
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M18-254

The M18-254 antibody has unknown specificity and can be used as an immunoglobulin isotype control.

553476 Rev. 12
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553476 Rev.12

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.