PE Mouse Anti-Human IL-8

BD FastImmune™ PE Mouse Anti-Human IL-8

Name: PE Mouse Anti-Human IL-8
Brand: PE Mouse Anti-Human IL-8
SKU: 340510

Clone AS14 (also known as AS14) (RUO (GMP))

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD FastImmune™

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Mouse

Application: Flow cytometry

Vol. Per Test: 20 μL

Entrez Gene ID: 3576

RRID: AB_400433

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

The FITC and PE conjugates are each supplied in 1.0 mL of PBS containing gelatin and 0.1% sodium azide. Please refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340510 Rev. 1

Antibody Details

AS14

Anti-Hu–IL-8, clone AS14, is derived from the hybridization of mouse P3X63Ag8 cells with spleen cells from BALB/c mice immunized with recombinant human IL-8.

Anti-Human Interleukin-8 (Anti-Hu–IL-8) recognizes a nonglycosylated protein of approximately 8 kilodaltons (kd).

340510 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

340510 Rev.1

Citations & References

View product citations for antibody "340510" on CiteAb

Development References (6)

Cassatella MA, Meda L, Bonora S, et al. Interleukin 10 (IL-10) inhibits the release of proinflammatory cytokines from human polymorphonuclear leukocytes. Evidence for an autocrine role of tumor necrosis factor and IL-1 β in mediating the production of IL-8 triggered by lipopolysaccharide. J Exp Med. 1993; 178:2207-2211. (Biology).
DeForge LE, Kenney JS, Jones ML, et al. Biphasic production of IL-8 in lipopolysaccharide (LPS)-stimulated human whole blood. Separation of LPS- and cytokine-stimulated components using anti-tumor necrosis factor and anti-IL-1 antibodies. J Immunol. 1992; 148:2133-2141. (Biology).
Hebert CA, Luscinskas FW, Kiely JM, et al. Endothelial and leukocyte forms of IL-8. Conversion by thrombin and interactions with neutrophils. J Immunol. 1990; 145(9):3033-3040. (Biology). View Reference
Larsen CG, Anderson AO, Appella E, Oppenheim JJ, Matsushima K. The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes. Science. 1989; 243(4897):1464-1466. (Biology). View Reference
Leonard EJ, Skeel A, Yoshimura T, Noer K, Kutvirt S, Van Epps D. Leukocyte specificity and binding of human neutrophil attractant/activation protein-1. J Immunol. 1990; 144(41323):1323-1330. (Biology). View Reference
Yoshimura T, Matsushima K, Oppenheim JJ, et al. Neutrophil chemotactic factor produced by lipopolysaccharide (LPS)-stimulated human blood mononuclear leukocytes: Partial characterization and separation from interleukin 1(IL-1). J Immunol. 1987; 139:788-793. (Biology).

View All (6)

340510 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.