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      PE Mouse Anti-Human IL-8
      Product Details
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      BD FastImmune™
      Human
      Mouse BALB/c IgG1, κ
      Mouse
      Flow cytometry
      20 μL
      3576
      Phosphate buffered saline with gelatin and 0.1% sodium azide.
      RUO (GMP)


      Preparation And Storage

      The FITC and PE conjugates are each supplied in 1.0 mL of PBS containing gelatin and 0.1% sodium azide. Please refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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      340510 Rev. 1
      Antibody Details
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      AS14

      Anti-Hu–IL-8, clone AS14, is derived from the hybridization of mouse P3X63Ag8 cells with spleen cells from BALB/c mice immunized with recombinant human IL-8.

      Anti-Human Interleukin-8 (Anti-Hu–IL-8) recognizes a nonglycosylated protein of approximately 8 kilodaltons (kd).

      340510 Rev. 1
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      340510 Rev.1
      Citations & References
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      Development References (35)

      1. Baggiolini M, Walz A, Kunkel SL. Neutrophil activating peptide 1/ IL-8, a novel cytokine that activates neutrophils. J Clin Invest. 1989; 84:1045-1049. (Biology).
      2. Bazzoni F, Cassatella MA, Rossi F, et al. Phagocytosing neutrophils produce and release high amounts of the neutrophil-activating peptide 1/interleukin 8. J Exp Med. 1991; 173:771-774. (Biology).
      3. Cassatella MA, Meda L, Bonora S, et al. Interleukin 10 (IL-10) inhibits the release of proinflammatory cytokines from human polymorphonuclear leukocytes. Evidence for an autocrine role of tumor necrosis factor and IL-1 β in mediating the production of IL-8 triggered by lipopolysaccharide. J Exp Med. 1993; 178:2207-2211. (Biology).
      4. Clore GM, Appella E, Yammada M, et al. Determination of the secondary structure of interleukin-8 by nuclear magnetic resonance spectroscopy. J Biol Chem. 1989; 264:18907-18911. (Biology).
      5. DeForge LE, Kenney JS, Jones ML, et al. Biphasic production of IL-8 in lipopolysaccharide (LPS)-stimulated human whole blood. Separation of LPS- and cytokine-stimulated components using anti-tumor necrosis factor and anti-IL-1 antibodies. J Immunol. 1992; 148:2133-2141. (Biology).
      6. Deforge LE, Remick DG. Kinetics of TNF, IL-6 and IL-8 gene expression in LPS-stimulated human whole blood. Biochem Biophys Res Commun. 1991; 174:18-24. (Biology).
      7. Francia di Celle P, Carbone A, Marchis D, et al. Cytokine gene expression in B-chronic lymphocytic leukemia: Evidence of constitutive interleukin-8 (IL-8) mRNA expression and secretion of biologically active IL-8 protein. Blood. 1994; 84:220-228. (Biology).
      8. Fujishima S, Nakamura H, Yasuhiro W, et al. Cell-associated IL-8 in human blood monocytes: Analysis by flow cytometry. Cytometry. 1996; 24:382-389. (Biology).
      9. Gimbrone MA, Obin MS, Brock AF, et al. Endothelial interleukin-8: a novel inhibitor of leukocyte-endothelial interactions. Science. 1989; 246:1601-1603. (Biology).
      10. Hebert CA, Luscinskas FW, Kiely JM, et al. Endothelial and leukocyte forms of IL-8. Conversion by thrombin and interactions with neutrophils. J Immunol. 1990; 145(9):3033-3040. (Biology). View Reference
      11. Jeannin P, Delneste Y, Gosset P, et al. Histamine induces interleukin-8 secretion by endothelial cells. Blood. 1994; 84:2229-2233. (Biology).
      12. Johnson K, Aarden L, Choi Y, et al. The proinflammatory cytokine response to coagulation and endotoxin in whole blood. Blood. 1996; 87:5051-5060. (Biology).
      13. Koch AE, Kunkel SL, Burrows JC, et al. Synovial tissue macrophage as a source of the chemotactic cytokine IL-8. J Immunol. 1991; 147:2187-2195. (Biology).
      14. Koch AE, Polverini PJ, Kunkel SL, et al. Interleukin-8 as a Macrophage-Derived Mediator of Angiogenesis. Science. 1996; 184:191-201. (Biology).
      15. Kunkel SL, Strieter RM, Lindley IJD. Chemokines: new ligands, receptors and activities. Immunol Today. 1995; 16:559-561. (Biology).
      16. Larsen CG, Anderson AO, Appella E, Oppenheim JJ, Matsushima K. The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes. Science. 1989; 243(4897):1464-1466. (Biology). View Reference
      17. Leonard EJ, Skeel A, Yoshimura T, Noer K, Kutvirt S, Van Epps D. Leukocyte specificity and binding of human neutrophil attractant/activation protein-1. J Immunol. 1990; 144(41323):1323-1330. (Biology). View Reference
      18. Liebler JM, Kunkel SL, Burdick MD, et al. Production of IL-8 and monocyte chemotactic peptide-1 by peripheral blood monocytes. Disparate responses to phytohemagglutinin and lipopolysaccharide. J Immunol. 1994; 152:241-249. (Biology).
      19. Martich GD, Danner RL, Ceska M, et al. Detection of interleukin 8 and tumor necrosis factor in normal humans after intravenous endotoxThe effect of anti-inflammatory agents. J Exp Med. 1991; 173:1021-1024. (Biology).
      20. Matsushima K, Morishita K, Yoshimura T, et al. Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by Interleukin 1 and tumor necrosis factor. J Exp Med. 1988; 167:1883-1893. (Biology).
      21. Oppenheim JJ, Zachariae COC, Mukaida N, et al. Properties of the novel proinflammatory supergene intercrine cytokine family. Ann Rev Immunol. 1991; 9:617-648. (Biology).
      22. Rajarathnam K, Skyes BD, Kay CM, et al. Neutrophil activation by monomeric interleukin 8. Science. 1994; 264:90-92. (Biology).
      23. Roth M, Nauck M, Yousefi S, et al. Platelet-activating factor exerts mitogenic activity and stimulates expression of interleukin 6 and interleukin 8 in human lung fibroblasts via binding to its functional receptor. J Exp Med. 1996; 184:191-201. (Biology).
      24. Schadendorf D, Möller A, Algermissen B, et al. IL-8 produced by human malignant melanoma cells in vitro is an essential autocrine growth factor. J Immunol. 1993; 151:2667-2675. (Biology).
      25. Schroder J-M, Sticherling M, Henneicke HH, et al. IL-1 α or tumor necrosis factor-α stimulate release of three NAP-1/IL-8-related neutrophil chemotactic proteins in human dermal fibroblasts. J Immunol. 1990; 144:2223-2232. (Biology).
      26. Schroder JM, Mrowietz U, Christophers E. Purification and partial biologic characterization of a human lymphocyte-derived peptide with potent neutrophil-stimulating activity. J Immunol. 1988; 140:3534-3540. (Biology).
      27. Smyth MJ, Zachariae COC, Norihisa Y, et al. IL-8 gene expression and production in human peripheral blood lymphocyte subsets. J Immunol. 1991; 146:3815-3823. (Biology).
      28. Standiford TJ, Strieter RM, Chensue SW, et al. IL-4 inhibits the expression of IL-8 from stimulated human monocytes. J Immunol. 1990; 145:1435-1439. (Biology).
      29. Strieter RM, Kunkel SL, Showell HJ, et al. Endothelial cell gene expression of a neutrophil chemotactic factor by TNF- α, LPS, and IL-1 β. Science. 1989; 243:1467-1469. (Biology).
      30. Strieter RM, Phan SH, Showell HJ, et al. Monokine-induced neutrophil chemotactic factor gene expression in human fibroblasts. J Biol Chem. 1989; 264:10621-10626. (Biology).
      31. Uguccioni M, Meliconi R, Nesci S, et al. Elevated interleukin-8 serum concentrations in β -thalassemia and graft-versus-host disease. Blood. 1993; 81:2252-2256. (Biology).
      32. Van Zee KJ, DeForge LE, Fischer E, et al. IL-8 in septic shock, endotoxemia and after IL-1 administration. J Immunol. 1991; 146:3478-3482. (Biology).
      33. Yoshimura T, Matsushima K, Oppenheim JJ, et al. Neutrophil chemotactic factor produced by lipopolysaccharide (LPS)-stimulated human blood mononuclear leukocytes: Partial characterization and separation from interleukin 1(IL-1). J Immunol. 1987; 139:788-793. (Biology).
      34. Yoshimura T, Matsushima K, Oppenheim JJ, et al. Neutrophil chemotactic factor produced by lipopolysaccharide (LPS)-stimulated human blood mononuclear leukocytes: Partial characterization and separation from interleukin 1(IL-1). J Immunol. 1987; 139:788-793. (Biology).
      35. de Waal Malefyt R, Abrams J, Bennett B, et al. Interleukin 10 (IL-10) inhibits cytokine synthesis by human monocytes: An autoregulatory role of IL-10 produced by monocytes. J Exp Med. 1991; 174:1209-1220. (Biology).
      View All (35) View Less
      340510 Rev. 1

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      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.