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Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
The CD8β antibody, clone 2ST8.5H7, is derived from the hybridization NS-1 mouse tumor cells with splenocytes from Balb/c mice immunized with CD8+ suppressor T-cell clone termed 20a6.
The CD8β antibody recognizes an epitope formed by the combination of CD8 alpha and beta chains. The majority of peripheral blood CD8+ T lymphocytes expresses an α/β heterodimer (32, 30 kilodaltons (kDa)), while CD8+CD16+ natural killer (NK) lymphocytes and CD8+ T-cell receptor (TCR)-γ/δ+ T lymphocytes express only an α/α homodimer. CD8β can therefore be used to selectively bind to CD8+ T cells while excluding CD8+ NK cells. The CD8 antigenic determinant binds to class I major histocompatibility (MHC) molecules, resulting in increased adhesion between the CD8+ T lymphocytes and target cells. Binding of the CD8 antigen to class I MHC molecules enhances the activation of resting T lymphocytes. The CD8 antigen is coupled to a protein tyrosine kinase, p56lck. The CD8:p56lck complex can play a role in T-lymphocyte activation through mediation of the interactions between the CD8 antigen and the CD3/TCR complex.
Development References (30)
-
Anderson P, Blue ML, Morimoto C, Schlossman SF. Cross-linking of T3 (CD3) with T4 (CD4) enhances the proliferation of resting T lymphocytes. J Immunol. 1987; 139:678-682. (Biology).
-
Campbell MJ, Scott J, Maecker HT, Park JW, Esserman LJ. Immune dysfunction and micrometastases in women with breast cancer. Breast Cancer Res Treat. 2005; 91:163-171. (Biology).
-
Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
-
Chang HC, Tan K, Ouvang J, et al. Structural and mutational analyses of a CD8αβ heterodimer and comparison with the CD8αα homodimer. Immunity. 2005; 23:661-671. (Biology).
-
Clinical and Laboratory Standards Institute. 2005. (Biology).
-
Cosimi AB. Anti-T-cell monoclonal antibodies in transplantation therapy. Trans Proc. 1983; XV:1889-1892. (Biology).
-
Eichmann K, Johnson J, Falk I, Emmrich F. Effective activation of resting mouse T lymphocytes by cross-linking submitogenic concentrations of the T-cell antigen receptor with either Lyt-2 or L3T4. Eur J Immunol. 1987; 17:643-650. (Biology).
-
Engleman EG, Benike CJ, Evans RL. Circulating antigen-specific suppressor T cells in a healthy woman: mechanism of action and isolation with a monoclonal antibody. Clin Res. 1981; 29:365A. (Biology).
-
Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man. J Exp Med. 1981; 154(1):193-198. (Biology). View Reference
-
Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology). View Reference
-
Gallagher PF, Fazekas de St. Groth B, Miller JFAP. CD4 and CD8 molecules can physically associate with the same T-cell receptor. Proc Natl Acad Sci USA. 1989; 86:10044-10048. (Biology).
-
Gatenby PA, Kansas GS, Xian CY, Evans RL, Engleman EG. Dissection of immunoregulatory subpopulations of T lymphocytes within the helper and suppressor sublineages in man. J Immunol. 1982; 129(5):1997-2000. (Biology). View Reference
-
Jacobson MA, Maecker HT, Orr PL, et al. Results of a cytomegalovirus (CMV)-specific CD8+/interferon-γ+ cytokine flow cytometry assay correlate with clinical evidence of protective immunity in patients with AIDS and CMV retinitis. J Infec Dis. 2004; 189:1362-1373. (Biology).
-
Joh T, Yamada Y, Seto M, Kamihira S, Tomonaga M. Expression of CD8β and alteration of cell surface phenotype in adult T-cell leukaemia cells. Br J Haematol. 1997; 98:151-156. (Biology).
-
Kienzle N, Baz A, Kelso A. Profiling the CD8low phenotype, an alternative career choice for CD8 T cells during primary differentiation. Immunol Cell Biol. 2004; 82:75-83. (Biology).
-
Konno A, Okada K, Mizuno K, et al. CD8α α memory effector T cells descend directly from clonally expanded CD8α +β high TCRα β T cells in vivo. Blood. 2002; 100:4090-4097. (Biology).
-
Kotzin BL, Benike CJ, Engleman EG. Induction of immunoglobulin-secreting cells in the allogeneic mixed leukocyte reaction: regulation by helper and suppressor lymphocyte subsets in man. J Immunol. 1981; 127(9):931-935. (Biology). View Reference
-
Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
-
Ledbetter JA, Frankel AE, Herzenberg. Human Leu T-cell differentiation antigens: quantitative expression on normal lymphoid cells and cell lines. In: Hammerling G, Hammerling U, Kearney J, ed. Monoclonal Antibodies and T Cell Hybridomas: Perspectives and Technical News. New York: Elsevier/North Holland Biomedical Press; 1981:16-22.
-
Maecker HT, Maino VC. Analyzing T-cell responses to cytomegalovirus by cytokine flow cytometry. Hum Immunol. 2004; 65:493-499. (Biology).
-
Mazzarino P, Pietra G, Vacca P, et al. Identification of effector-memory CMV-specific T lymphocytes that kill CMV-infected target cells in an HLA-E–restricted fashion. Eur J Immunol. 2005; 35:3240-3247. (Biology).
-
Moebius U. Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV. White Cell Differentiation Antigens. New York: Oxford University Press; 1989:342-343.
-
Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
-
Rudd CE, Burgess KE, Barber EK, Schlossman SF. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:326-327.
-
Schmitz JE, Forman MA, Lifton MA, et al. Expression of the CD8α β-heterodimer on CD8+ T lymphocytes in peripheral blood lymphocytes of human immunodeficiency virus- and human immunodeficiency virus+ individuals. Blood. 1998; 92:198-206. (Biology).
-
Suni MA, Ghanekar SA, Houck DW, et al. CD4+CD8dim T lymphocytes exhibit enhanced cytokine expression, proliferation and cytotoxic activity in response to HCMV and HIV-1 antigens. Eur J Immunol. 2001; 31:2512-2520. (Biology).
-
Terry LA, DiSanto JP, Small TN, Flomenberg N. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:345-346.
-
Tu W, Chen S, Sharp M, et al. Persistent and selective deficiency of CD4+T cell immunity to cytomegalovirus in immunocompetent young children. J Immunol. 2004; 172:3260-3267. (Biology).
-
Werwitzke S, Drescher BE, Schmidt RE, Witte T. CD8+ T cell populations in common variable immunodeficiency. Immunobiology. 2000; 202:168-171. (Biology).
-
Werwitzke S, Tiede A, Drescher BE, Schmidt RE, Witte T. CD8 β/CD28 expression defines functionally distinct populations of peripheral blood T lymphocytes. Clin Exp Immunol. 2003; 133:334-343. (Biology).
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