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Purified Mouse Anti-TIF2
Purified Mouse Anti-TIF2

Western blot analysis of TIF2 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-TIF2 antibody.

Purified Mouse Anti-TIF2

Immunofluorescence staining of C3H/10T1/2 cells (Mouse embryonic fibroblasts; ATCC CCL-226).

Western blot analysis of TIF2 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-TIF2 antibody.

Immunofluorescence staining of C3H/10T1/2 cells (Mouse embryonic fibroblasts; ATCC CCL-226).

Product Details
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BD Transduction Laboratories™
Transcriptional Intermediary Factor-2
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human TIF2 aa. 959-1067
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
160 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610985 Rev. 1
Antibody Details
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Nuclear receptors (NRs) are a family of ligand-inducible transcription factors that trigger complex events during development, differentiation, and homeostasis. They respond to the binding of steroid and thyroid hormones, retinoids, and vitamins. NRs contain an activation domain AF-1, which constitutively activates transcription. A second activation domain, AF-2, responds to ligand binding. Transcriptional interference/squelching between the AFs of steroid receptors first suggested the existence of Transcriptional Intermediary Factors (TIFs). TIFs mediate AF activity to the transcriptional machinery and chromatin template.  TIF2 interacts directly with the ligand binding domains of several NRs in an  agonist and AF-2-integrity-dependent manner. It has autonomous activation  function, relieves interference between NRs, and has been shown to enhance AF-2 activity. TIF2 contains an NR interaction domain (NID) and two autonomous activation functions (AD1 and AD2). AD1 is identical to the TIF2 CBP interaction domain (CID). Thus, it is thought that TIF2 links NR AF2 activity and CBP via AD1 and functions as a transcriptional mediator through unknown CBP-independent mechanisms via AD2.

610985 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
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Citations & References
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Development References (4)

  1. Heery DM, Kalkhoven E, Hoare S, Parker MG. A signature motif in transcriptional co-activators mediates binding to nuclear receptors. Nature. 1997; 387(6634):733-736. (Biology). View Reference
  2. Muller JM, Metzger E, Greschik H. The transcriptional coactivator FHL2 transmits Rho signals from the cell membrane into the nucleus. EMBO J. 2002; 21(14):736-748. (Biology: Western blot). View Reference
  3. Voegel JJ, Heine MJ, Zechel C, Chambon P, Gronemeyer H. TIF2, a 160 kDa transcriptional mediator for the ligand-dependent activation function AF-2 of nuclear receptors. EMBO J. 1996; 15(14):3667-3675. (Biology). View Reference
  4. Xu J, Qiu Y, DeMayo FJ, Tsai SY, Tsai MJ, O'Malley BW. Partial hormone resistance in mice with disruption of the steroid receptor coactivator-1 (SRC-1) gene. Science. 1998; 279(5358):1922-1925. (Biology). View Reference
View All (4) View Less
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

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