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Purified Mouse Anti-Plectin
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human Plectin aa. 3062-3184
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
400/500 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611348 Rev. 1
Antibody Details
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Plectin is an intermediate filament-binding protein that acts as a cross-linking elementin the cytoskeleton. Sequence analysis reveals that plectin is dumbbell shaped and contains a long central α-helical coiled-coil rod flanked by globular domains. Plectinhas six C-terminal repeats that exhibit a tandemly repeated amino acid sequence motif that is characteristic of intermediate filament-associated proteins, such as BPAG1 and desmoplakin. Plectin associates with the sarcolemma, nuclear membrane, and intermyofibrillar network in muscle and with hemidesmosmes in skin. These localizations may be a result of plectin binding to a variety of cytoskeletal proteins including laminin B, microtubule-associated proteins, and α-spectrin. In addition, plectin interacts with G-actin in a PIP2-dependent manner and with actin stress fibers viaits N-terminal actin-binding domain. Patients with epidermolysis bullosa simplex, adisease that results in structural and functional abnormalities in muscle and skin, have no plectin expression in muscle tissues and deficient plectin levels in skin. Thus, plectin cytoskeletal association may be critical for the structural integrity of muscle and skin.

611348 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
611348 Rev.1
Citations & References
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Development References (4)

  1. Andra K, Nikolic B, Stocher M, Drenckhahn D, Wiche G. Not just scaffolding: plectin regulates actin dynamics in cultured cells. Genes Dev. 1998; 12(21):3442-3451. (Biology). View Reference
  2. Banwell BL, Russel J, Fukudome T, Shen XM, Stilling G, Engel AG. Myopathy, myasthenic syndrome, and epidermolysis bullosa simplex due to plectin deficiency. J Neuropathol Exp Neurol. 1999; 58(8):832-846. (Biology). View Reference
  3. Liu CG, Maercker C, Castanon MJ, Hauptmann R, Wiche G. Human plectin: organization of the gene, sequence analysis, and chromosome localization (8q24). Proc Natl Acad Sci U S A. 1996; 93(9):4278-4283. (Biology). View Reference
  4. Wiche G, Krepler R, Artlieb U, Pytela R, Denk H. Occurrence and immunolocalization of plectin in tissues. J Cell Biol. 1983; 97(3):887-901. (Biology). View Reference
View All (4) View Less
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

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