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Purified Mouse Anti-PIN
Product Details
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BD Transduction Laboratories™
Protein Inhibitor of nNOS
Rat (QC Testing), Human (Tested in Development)
Mouse IgG1
Rat PIN aa. 1-89
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required), Immunoprecipitation (Not Recommended)
10 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610726 Rev. 1
Antibody Details
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PIN (protein inhibitor of nNOS) is a small protein of 89 amino acids initially described as a light chain subunit of dynein and as an inhibitor of the neuronal nitric oxide synthase isoform (nNOS). In vitro, PIN has been reported to bind to a unique nNOS domain encompassing amino acids 163-245. PIN inhibits nNOS activity and blocks the formation of the active nNOS dimer. Although ubiquitously expressed, PIN levels are reportedly highest in brain and testis. Immunolocalization studies in Drosophila melanogaster detected a cytoplasmic distribution of PIN. Partial-loss-of-function of PIN resulted in morphologic and developmental changes in the bristles, wings, and female sterility of  D. melanogaster. Deletion of PIN resulted in lethality with the characteristic morphology of apoptotic cells.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610726 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610726 Rev.1
Citations & References
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Development References (2)

  1. Dick T, Ray K, Salz HK, Chia W. Cytoplasmic dynein (ddlc1) mutations cause morphogenetic defects and apoptotic cell death in Drosophila melanogaster. Mol Cell Biol. 1996; 16(5):1966-1977. (Biology). View Reference
  2. Jaffrey SR, Snyder SH. PIN: an associated protein inhibitor of neuronal nitric oxide synthase. Science. 1996; 274(5288):774-776. (Biology). View Reference
610726 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.