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Purified Mouse Anti-eIF-4γ
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Dog (Tested in Development)
Mouse IgG1
Human eIF-4γ aa. 1217-1386
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
220 kDa
250 µg/ml
AB_397893
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610536 Rev. 1
Antibody Details
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40/eIF-4γ

eIF-4 proteins are required for mRNA recognition and acceleration of translation during protein synthesis. This group of proteins consists of the RNA helicase, eIF-4A; the RNA-binding protein, eIF-4B; and the cap binding proteins, eIF-4E and eIF-4γ. eIF-4γ, also referred to as p220, is a 1396 amino acid polypeptide that migrates as a 220kDa. eIF-4γ has several potential glycosylation and phosphorylation sites that could account for its anomalous electrophoretic mobility. In addition, eIF-4γ is rapidly degraded in picornavirus-infected cells due to its content of multiple PEST sequences.

610536 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610536 Rev.1
Citations & References
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Development References (3)

  1. Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J Biol Chem. 1996; 271(2):623-626. (Biology). View Reference
  2. Kedersha N, Chen S, Gilks N. Evidence that ternary complex (eIF2-GTP-tRNA(i)(Met))-deficient preinitiation complexes are core constituents of mammalian stress granules. Mol Biol Cell. 2002; 13(1):195-210. (Clone-specific: Immunofluorescence). View Reference
  3. Yan R, Rychlik W, Etchison D, Rhoads RE. Amino acid sequence of the human protein synthesis initiation factor eIF-4 gamma. J Biol Chem. 1992; 267(32):23226-23231. (Biology). View Reference
610536 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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