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Purified Mouse Anti-DAP3
Purified Mouse Anti-DAP3

Western blot analysis of DAP3 on a SW-13 cell lysate (Human adrenal gland carcinoma; ATCC CCL-105). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-DAP3 antibody.

Purified Mouse Anti-DAP3

Immunofluorescence staining of human endothelial cells.

Western blot analysis of DAP3 on a SW-13 cell lysate (Human adrenal gland carcinoma; ATCC CCL-105). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-DAP3 antibody.

Immunofluorescence staining of human endothelial cells.

Product Details
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BD Transduction Laboratories™
Death Associated Protein-3
Human (QC Testing), Mouse, Rat, Frog (Tested in Development)
Mouse IgG1
Human DAP3 aa. 98-248
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
46 kDa
250 µg/ml
AB_397989
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610662 Rev. 1
Antibody Details
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10/DAP3

Chronic exposure to interferons inhibits cell proliferation and results in cell death. The gene for the Death Associated Protein-3 (DAP3) was identified using a novel approach named Technical Knockout. Briefly, HeLa cells were tranfected with an antisense cDNA expression library, then exposed to interferon-γ. Surviving cells were rescued and the anti-sense "protecting" genes were isolated. Several genes were identified, including one for DAP3. The DAP3 gene was found widely expressed and, as predicted, DAP3 antisense mRNA protected HeLa cells from the killing effects of interferon-γ. Thus, this novel protein lies somewhere in the signaling downstream of interferon-γ induced cell death.

610662 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610662 Rev.1
Citations & References
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Development References (4)

  1. Fulda S, Debatin KM. IFNgamma sensitizes for apoptosis by upregulating caspase-8 expression through the Stat1 pathway. Oncogene. 2002; 21(15):2295-2308. (Biology: Western blot). View Reference
  2. Kim Y, Suh N, Sporn M, Reed JC. An inducible pathway for degradation of FLIP protein sensitizes tumor cells to TRAIL-induced apoptosis. J Biol Chem. 2002; 277(25):22320-22329. (Biology: Western blot). View Reference
  3. Kissil JL, Deiss LP, Bayewitch M, Raveh T, Khaspekov G, Kimchi A. Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death. J Biol Chem. 1995; 270(46):27932-27936. (Biology). View Reference
  4. Pedersen IM, Kitada S, Schimmer A, et al. The triterpenoid CDDO induces apoptosis in refractory CLL B cells. Blood. 2002; 100(8):2965-2972. (Biology: Western blot). View Reference
View All (4) View Less
610662 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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