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Purified Rat Anti-Mouse CD90
Product Details
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BD Pharmingen™
Thy-1
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2c, κ
Mouse T-cell hybridoma C 6/68
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Activation, Apoptosis, Immunohistochemistry-zinc-fixed (Reported), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
0.5 mg/ml
AB_394554
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Other reported applications include in vitro T-cell activation induction of apoptosis, and immunohistochemical staining (IHC) of acetone-fixed frozen and zinc-fixed paraffin-embedded sections. IHC of formalin-fixed paraffinembedded sections is not recommended. For IHC of tissue expressing the Thy-1. alloantigen, we recommend the use of anti-mouse CD90.2 mAb 53-2.1 (Cat. no. 550543) in our special formulation for immunohistochemistry.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553016 Rev. 13
Antibody Details
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G7

The G7 antibody reacts with the Thy-1.1 and Thy-1.2 alloantigens (CD90.1 and CD90.2, respectively) on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), and neurons, but not B lymphocytes, of all mouse strains.  CD90 is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction.  In addition, there is evidence that CD90 mediates adhesion of thymocytes to thymic stroma.  Soluble G7 mAb stimulates murine T-cell proliferation and IL-2 secretion, via signalling through the TCR/CD3 complex.  mAb G7 has been reported to promote apoptosis of thymocytes and CTL clones, as determined by morphological and biochemical criteria.  The Thy-1-triggered thymocyte death was found to be developmentally regulated and targeted mainly within the CD4+CD8+CD3- and CD4+CD8+CD3lo cultured immature thymocytes.  G7 antibody does not react with Thy-1 (CD90) on rat thymocytes.

553016 Rev. 13
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553016 Rev.13
Citations & References
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Development References (10)

  1. Gunter KC, Malek TR, Shevach EM. T cell-activating properties of an anti-Thy-1 monoclonal antibody. Possible analogy to OKT3/Leu-4. J Exp Med. 1984; 159(3):716-730. (Immunogen: Activation). View Reference
  2. He HT, Naquet P, Caillol D, Pierres M. Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism. J Exp Med. 1991; 173(2):515-518. (Biology). View Reference
  3. Hueber AO, Raposo G, Pierres M, He HT. Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism. J Exp Med. 1994; 179(3):785-796. (Clone-specific: Apoptosis). View Reference
  4. Kroczek RA, Gunter KC, Germain RN, Shevach EM. Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes. Nature. 1986; 322(6075):181-184. (Biology). View Reference
  5. Kruisbeek AM, Shevach EM. Proliferative assays for T cell function. Curr Protoc Immunol. 2004; 3:3.12.1-3.12.14. (Clone-specific: Activation). View Reference
  6. Nitta H, Munger W, Wilson E. Improved in situ immunodetection of leukocytes paraffin embedded mouse spleen. Cell Vis. 1997; 4:73-80. (Clone-specific: Immunohistochemistry).
  7. Sugiyama E, Cantagrel A, Reno T, Stafford-Brady F, Yeh ET, Bonventre JV. Selection of T cell receptor expression mutants through the functionally linked Ly-6A. Cell Immunol. 1990; 130(2):271-280. (Clone-specific: Apoptosis). View Reference
  8. Tentori L, Pardoll DM, Zuniga JC . Proliferation and production of IL-2 and B cell stimulatory factor 1/IL-4 in early fetal thymocytes by activation through Thy-1 and CD3. J Immunol. 1988; 140(4):1089-1094. (Clone-specific: Activation). View Reference
  9. Ucker DS, Meyers J, Obermiller PS. Activation-driven T cell death. II. Quantitative differences alone distinguish stimuli triggering nontransformed T cell proliferation or death. J Immunol. 1992; 149(5):1583-1592. (Clone-specific: Apoptosis, Induction). View Reference
  10. Williams AF, Gagnon J. Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin. Science. 1982; 216(4547):696-703. (Biology). View Reference
View All (10) View Less
553016 Rev. 13

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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