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      RY586 Mouse Anti-Human CD179a (VpreB1)
      RY586 Mouse Anti-Human CD179a (VpreB1)

      Flow cytometric analysis of surface CD179a (VpreB1) expression on Human 293F cells.  Human 293F untransfected (dashed line histogram) and transfected (solid line histogram) cells were surface stained with BD Horizon™ RY586 Mouse Anti-Human CD179a (VpreB1) antibody (Cat. No. 570347/570417). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing CD179a (VpreB1) expression were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.

      RY586 Mouse Anti-Human CD179a (VpreB1)

      Flow cytometric analysis of surface CD179a (VpreB1) expression on Human 293F cells.  Human 293F untransfected (dashed line histogram) and transfected (solid line histogram) cells were surface stained with BD Horizon™ RY586 Mouse Anti-Human CD179a (VpreB1) antibody (Cat. No. 570347/570417). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing CD179a (VpreB1) expression were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.

      Product Details
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      BD Horizon™
      CD179a; IGI; IGVPB; VPREB; VPREB1; VpreB; VpreB1; pre-B lymphocyte 1; v(pre)B protein
      Human (QC Testing)
      Mouse BALB/c IgG1, κ
      Human VpreB/λ5 Recombinant Protein
      Flow cytometry (Routinely Tested)
      5 µl/test
      VII 70514
      7441
      AB_3685679
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. CF™ is a trademark of Biotium, Inc.
      9. For U.S. patents that may apply, see bd.com/patents.
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      570417 Rev. 1
      Antibody Details
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      HSL96

      The HSL96 monoclonal antibody specifically recognizes CD179a which is also known as VpreB (V pre beta/V pre β). CD179a (VpreB1) is a ~18 kDa, Ig V domain-like protein that is encoded by VPREB1 (pre-B lymphocyte 1). This member of the immunoglobulin (Ig) gene superfamily is primarily expressed in the cytoplasm of normal pro-B and early pre-B cells and at low levels on the surface of early pre-B cells.  It is not expressed by normal mature circulating B cells or by other leucocyte populations. CD179a (VpreB1) associates noncovalently with CD179b (λ5) to form CD179a/CD179b (VpreB/ λ5), an Ig light chain-like structure called the surrogate light chain. Surrogate light chains are disulfide-linked to membrane-bound IgM heavy chains in association with signal transducer CD79a/CD79b heterodimers which together form the pre-B cell receptor (preBCR) complex. The preBCR plays a critical role in early B cell proliferation and differentiation. The HSL96 antibody can reportedly be used to stain cytoplasmic and cell surface CD179a (VpreB1).

      570417 Rev. 1
      Format Details
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      RY586
      The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
      altImg
      RY586
      Yellow-Green 561 nm
      564 nm
      586 nm
      570417 Rev.1
      Citations & References
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      View product citations for antibody "570417" on CiteAb

      Development References (6)

      1. Karasuyama H, Lebien TW, Copper MD, Clark EC. CD179 Workshop report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:112-115.
      2. Kiyokawa N, Sekino T, Matsui T, et al. Diagnostic importance of CD179a/b as markers of precursor B-cell lymphoblastic lymphoma.. Mod Pathol. 2004; 17(4):423-9. (Clone-specific: Flow cytometry). View Reference
      3. Matsuo Y, Drexler HG, Okochi A, Sugimoto A, Harashima A, Orita K. Characterization of human B cell-precursor leukemia and mature B-cell leukaemia/lymphoma cell lines: Expression and distribution of human pre-B-cell receptor. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:117-120.
      4. Tsuganezawa K, Kiyokawa N, Matsuo Y, et al. Expression profile of pre B-cell receptor components in acute lymphoblastic leukemia and its application to the diagnosis and classification of the disease. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:115-117.
      5. Tsuganezawa K, Kiyokawa N, Matsuo Y, et al. Flow cytometric diagnosis of the cell lineage and developmental stage of acute lymphoblastic leukemia by novel monoclonal antibodies specific to human pre-B-cell receptor.. Blood. 1998; 92(11):4317-24. (Immunogen: ELISA, Flow cytometry, Immunoprecipitation). View Reference
      6. Zola H, Swart B, Boumsell L, Mason DY. Human Leucocyte Differentiation Antigen nomenclature: update on CD nomenclature. Report of IUIS/WHO Subcommittee.. J Immunol Methods. 2003; 275(1-2):1-8. (Clone-specific: Flow cytometry). View Reference
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      570417 Rev. 1

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      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.