R718 Mouse Anti-Stat5 (pY694)
Flow cytometric analysis of Stat5 (PY694) expression in human CD4+ T lymphocytes. Untreated (dashed line histogram) or treated (solid line histogram) peripheral blood mononuclear cells (PBMC) from the BD Phosflow™ T Cell Kit Lyophilized Cells (Catalog No. 560760) were reconstituted in BD Pharmingen™ Stain Buffer (Cat. No. 554656) for 15 minutes at room temperature. These cells are fixed, permeabilized, and lyophilized PBMC that are either untreated or treated with stimulators [including phorbol 12-myristate 13-acetate (PMA), interferon-alpha (IFN-α), interleukin-2 (IL-2), IL-4, and IL-6]. The reconstituted cells were then washed and stained with BD Horizon™ BUV395 Mouse Anti-Human CD4 antibody (Cat. No. 564724) and BD Horizon™ R718 Mouse Anti-Human Stat1 (pY701) antibody (Cat. No. 566977/566978). Histograms showing Stat5 (pY694) expression were derived from CD4-positive gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Flow Cytometer System and FlowJo™ software.
BD Phosflow™ R718 Mouse Anti-Stat5 (pY694)
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