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Purified NA/LE Rat Anti-Mouse CD140a
Product Details
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BD Pharmingen™
Pdgfra; Pdgfr2; PDGF-R alpha; Platelet derived growth factor receptor alpha
Rat WF, also known as Wistar Furth IgG2a, κ
Mouse PDGF Receptor α chain
Flow cytometry (Routinely Tested)
1.0 mg/ml
18595
AB_2307390
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
562171 Rev. 1
Antibody Details
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APA5

The APA5 antibody monoclonal antibody specifically binds to CD140a, the Platelet-Derived Growth Factor Receptor alpha chain (PDGFR-a, PDGFR-α). CD140a is a receptor tyrosine kinase that is widely expressed on cells of mesenchymal origin in the embryo and adult. CD140a is expressed on several other cell types during embryonic development, but not on hematopoietic cells. CD140a binds to PDGF A and B chains, in contrast to PDGFR-b (CD140b) that binds only to the PDGF B chain.  Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms. Ligand binding to the PDGF Receptor induces the formation of receptor dimers (aa, ab, or bb), autophosphorylation, and internalization.  The APA5 antibody has been demonstrated to block binding of  PDGF-AA to PDGFR-a-expressing cells in vitro  and to block some PDGF-mediated developmental events in vivo.

562171 Rev. 1
Format Details
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NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
NA/LE
562171 Rev.1
Citations & References
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Development References (3)

  1. Fruttiger M, Calver AR, Krüger WH. PDGF mediates a neuron-astrocyte interaction in the developing retina. Neuron. 1996; 17(6):1117-1131. (Biology: Blocking, ELISA, Immunohistochemistry, Inhibition, Western blot). View Reference
  2. Heldin CH. Structural and functional studies on platelet-derived growth factor. EMBO J. 1992; 11(12):4251-4259. (Immunogen). View Reference
  3. Takakura N, Yoshida H, Kunisada T, Nishikawa S, Nishikawa SI. Involvement of platelet-derived growth factor receptor-alpha in hair canal formation. J Invest Dermatol. 1996; 107(5):770-777. (Clone-specific: Blocking). View Reference
562171 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.