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      Purified NA/LE Mouse Anti-Human IFN-α[2b]
      Product Details
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      BD Pharmingen™
      IFNa, IFNα
      Human (QC Testing)
      Mouse IgG1, κ
      Human IFN-α2b
      Intracellular staining (flow cytometry) (Routinely Tested), Neutralization (Tested During Development), (Reported)
      1.0 mg/ml
      AB_394252
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      551795 Rev. 4
      Antibody Details
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      7N4-1

      The 7N4-1 antibody reacts with human IFN-α2b and to a lesser extent with IFN-α7.  It does not react with IFN-α1 nor IFN-α4.  IFN-α2b is one of the three variants of IFN-α2 that have been isolated from human cell lines. IFN-α2b is the variant predominantly produced by human leukocytes.  Human IFN-α2b belongs to the IFN-α class of proteins also known as leukocyte interferons. IFN-α comprises a family of related but distinct proteins with molecular weights ranging from 16-27 kDa with antiviral, antiproliferative and immunomodulatory activities.  The IFN-α family is composed from as many as 14 different genes.  The immunogen used to generate the 7N4-1 hybridoma was E. coli-expressed recombinant human IFN-α2b. This is a neutralizing antibody.

      551795 Rev. 4
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      551795 Rev.4
      Citations & References
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      Development References (8)

      1. Dipaola M, Smith T, Ferencz-Biro K, Liao MJ, Testa D.. Interferon-alpha 2 produced by normal human leukocytes is predominantly interferon-alpha 2b. J Interferon Res. 1994; 14(6):325-332. (Biology). View Reference
      2. Green JA, Yeh TJ, Overall JC. Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons. J Clin Microbiol. 1980; 12(3):433-438. (Methodology: Neutralization). View Reference
      3. Lydon NB, Favre C, Bove S, et al. Immunochemical mapping of alpha-2 interferon. Biochemistry. 1985; 24(15):4131-4141. (Immunogen). View Reference
      4. Pestka S. Interferon from 1981 to 1986. Methods Enzymol. 1986; 119:3-14. (Biology). View Reference
      5. Pestka S. The human interferon alpha species and receptors. 2000; 55(4):254-287. (Biology). View Reference
      6. Pestka S. The human interferons—from protein purification and sequence to cloning and expression in bacteria: before, between, and beyond. Arch Biochem Biophys. 1983; 221(1):1-37. (Biology). View Reference
      7. Siegal FP, Kadowaki N, Shodell M, et al. The nature of the principal type 1 interferon-producing cells in human blood. Science. 1999; 284(5421):1835-1837. (Clone-specific: Immunocytochemistry (cytospins)). View Reference
      8. Vogel S, Friedman R, Hogan M. Measurement of antiviral activity induced by interferons a, b, and g.. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: John Wiley & Sons; 2007:6.9.1-6.9.15.
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      551795 Rev. 4

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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