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Purified NA/LE Mouse Anti-Human CD29
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Product Details
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BD Pharmingen™
Integrin β1 Chain
Human (QC Testing)
Mouse IgG2a, κ
Flow cytometry (Routinely Tested)
1.0 mg/ml
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
556047 Rev. 7
Antibody Details
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Reacts with the 130 kDa integrin β1 subunit, CD29, that is expressed following cell activation as a heterodimeric complex with one of nine distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. The HUTS-21 β1 epitope is weakly expressed on resting lymphocytes, but is upregulated by TPA, Ca++ ionophore, or another CD29 antibody, TS2/16. This upregulation is Mn++ dependent and not supported by other divalent cations such as Ca++ and/or Mg++. The VLA receptors have a broad cellular distribution and interact with ligands like VCAm-1 and MAdCAM-1 during cell adhesion. They also interact with extra-cellular matrices including collagen, laminin, fibronectin and others. HUTS-21 antibody enhances adherence of TPA-activated lymphocytes to fibronectin.

556047 Rev. 7
Format Details
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NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
556047 Rev.7
Citations & References
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Development References (5)

  1. Cabanas C, Hogg N. Ligand intercellular adhesion molecule 1 has a necessary role in activation of integrin lymphocyte function-associated molecule 1. Proc Natl Acad Sci U S A. 1993; 90(12):5838-5842. (Biology). View Reference
  2. Garcia-Gila M, Cabanas C, Garcia-Pardo A. Analysis of the activation state of alpha4beta1 integrin in human B cell lines derived from myeloma, leukemia or lymphoma. FEBS Lett. 1997; 418(3):337-340. (Biology). View Reference
  3. Gomez J, Garcia A, R-Borlado L, et al. IL-2 signaling controls actin organization through Rho-like protein family, phosphatidylinositol 3-kinase, and protein kinase C-zeta. J Immunol. 1997; 158(4):1516-1522. (Biology). View Reference
  4. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
  5. Luque A, Gomez M, Puzon W, Takada Y, Sanchez-Madrid F, Cabanas C. Activated conformations of very late activation integrins detected by a group of antibodies (HUTS) specific for a novel regulatory region (355-425) of the common beta 1 chain. J Biol Chem. 1996; 271(19):11067-11075. (Biology). View Reference
View All (5) View Less
556047 Rev. 7

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.