Purified Mouse Anti-Rabbit CD25

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BD Pharmingen™ Purified Mouse Anti-Rabbit CD25

Name: Purified Mouse Anti-Rabbit CD25
Brand: BD Pharmingen™
SKU: 551779

Clone Kei- alpha 1

(RUO)

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Product Details

Brand: BD Pharmingen™

Alternative Name: IL-2R alpha

Reactivity: Rabbit (QC Testing)

Isotype: Mouse BALB/c IgG2b, κ

Immunogen: Membrane preparation of the HTLV-1–transformed rabbit T-cell line F648b

Application: Flow cytometry (Routinely Tested), Blocking, Immunoprecipitation (Reported)

Concentration: 0.5 mg/ml

RRID: AB_394251

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Use of these products to measure activation antigens expressed on mononuclear cell subsets for the purpose of monitoring immunoregulatory status can fall under one or more claims of the following patents: US Patent Nos. 5,445,939, 5,656,446, 5,843,689; European Patent No. 319,543; Canadian Patent No. 1,296,622; Australian Patent No. 615,880; and Japanese Patent No. 2,769,156.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

551779 Rev. 9

Antibody Details

Kei- alpha 1

The Kei-α1 antibody reacts with the rabbit homolog of CD25, the interleukin-2 receptor alpha chain (IL-2Rα). mAb Kei-α1 recognizes specifically IL-2Rα and neither the IL-2R ß chain (CD122) nor the 25-kDa protein component of the rabbit high-affinity IL-2R complex. Based on flow cytometric analysis, fewer than 1% of resting peripheral blood T and B cells have been found to stain with Kei-α1, while a majority of PHA blasts show intense staining with Kei-α1. The HTLV-1-transformed rabbit cell lines, F648b and YR-1 both also show intense staining with Kei-α1. mAb Kei-α1 has been reported to block both high-and low-affinity IL-2 binding and also cell proliferation of rabbit PHA blasts induced by picomolar concentrations of IL-2.

551779 Rev. 9

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

551779 Rev.9

Citations & References

Development References (1)

Kotani M, Yamamura Y, Tsudo M, Tamatani T, Kitamura F, Miyasaka M. Generation of monoclonal antibodies to the rabbit interleukin-2 receptor alpha chain (CD25) and its distribution in HTLV-1-transformed rabbit T cells. Jpn J Cancer Res. 1993 July; 84(7):770-775. (Immunogen: Blocking, Immunoprecipitation). View Reference

551779 Rev. 9

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.