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PE Rat Anti-Mouse DCAR2 (Clec4b1)
Product Details
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BD Pharmingen™
C-type lectin domain family 4, member b1; Clec4b; DCAR; DCARbeta; Dcar2
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2b, κ
Mouse DCAR2 extracellular domain- Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2869933
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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566882 Rev. 1
Antibody Details
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7D2D3

The 7D2D3 monoclonal antibody specifically recognizes mouse Dendritic Cell Activating Receptor 2 (DCAR2), a C-type lectin encoded by the Clec4b1 gene. DCAR2 (Clec4b1) is a member of the dendritic cell immunoreceptor (DCIR) family, which contains four dendritic cell inhibitory receptors (DCIR1-4) and two activating receptors (DCAR1, 2). The extracellular region of DCAR2 (Clec4b1) has one C-type lectin carbohydrate recognition domain (CRD), that is highly similar to that of DCIR1, followed by a transmembrane region. Instead of a cytoplasmic Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM) that can transmit inhibitory signals, DCAR2 associates with the Immunoreceptor Tyrosine-based Activating Motif (ITAM)-containing adaptor molecule, Fc Receptor γ (FcRγ). DCAR2 (Clec4b1) is expressed on restricted subsets of conventional dendritic cells in bone marrow and skin-draining lymph nodes. C-type lectins bind to carbohydrates in a calcium-dependent manner, and this receptor may be involved in the regulation of immune responses to bacterial infections.

566882 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
496 nm, 566 nm
576 nm
566882 Rev.1
Citations & References
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View product citations for antibody "566882" on CiteAb

Development References (4)

  1. Hsu Y, Okada R, Nishimura T, Kawasaki N, Yamamoto K, Matsumoto N. DCIR3 and DCIR4 are co-expressed on inflammatory and patrolling monocytes.. Biochem Biophys Res Commun. 2017; 494(3-4):440-445. (Clone-specific: Flow cytometry). View Reference
  2. Kanazawa N, Tashiro K, Inaba K, Miyachi Y. Dendritic cell immunoactivating receptor, a novel C-type lectin immunoreceptor, acts as an activating receptor through association with Fc receptor gamma chain.. J Biol Chem. 2003; 278(35):32645-52. (Biology). View Reference
  3. Kishimoto A, Watanabe M, Terauchi K, et al. Ubiquitous versus restricted expression of the two mouse dendritic cell C-type lectin receptors, DCIR1 and DCAR2, among myeloid cells.. Biochem Biophys Res Commun. 2015; 467(2):383-8. (Immunogen: Flow cytometry). View Reference
  4. Toyonaga K, Torigoe S, Motomura Y, et al. C-Type Lectin Receptor DCAR Recognizes Mycobacterial Phosphatidyl-Inositol Mannosides to Promote a Th1 Response during Infection.. Immunity. 2016; 45(6):1245-1257. (Biology). View Reference
View All (4) View Less
566882 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.