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PE Mouse Anti-Pig CD3ε
PE Mouse Anti-Pig CD3ε

Multicolor flow cytometric analysis of CD3 expression on pig peripheral blood lymphocytes. Pig whole blood was stained simultaneously with PE Mouse Anti-Pig CD3ε antibody (Cat. No. 561485) and Alexa Fluor® 647 Mouse Anti-Pig CD4 antibody (Cat. No. 561472). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). A two-color flow cytometric dot plot showing the correlated expression of CD3 versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Multicolor flow cytometric analysis of CD3 expression on pig peripheral blood lymphocytes. Pig whole blood was stained simultaneously with PE Mouse Anti-Pig CD3ε antibody (Cat. No. 561485) and Alexa Fluor® 647 Mouse Anti-Pig CD4 antibody (Cat. No. 561472). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). A two-color flow cytometric dot plot showing the correlated expression of CD3 versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Product Details
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BD Pharmingen™
CD3 epsilon subunit; CD3e; T-cell surface glycoprotein CD3 epsilon chain
Pig (QC Testing)
Mouse BALB/c IgG2a, κ
Pig peripheral blood mononuclear cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_10679355
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
561485 Rev. 1
Antibody Details
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BB23-8E6-8C8

The BB23-8E6-8C8 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex.  It recognizes all CD4+ and most CD8+ peripheral blood T lymphocytes, most thymocytes and phytohemagglutinin-stimulated blasts, and subsets of spleen and Peyer's patch lymphocytes. BB23-8E6-8C8 is a immunoglobulin isotype switch variant of the BB23-8E6 clone. This isotype-switch variant induces a proliferative response of peripheral blood mononuclear cells. The epitope recognized by BB23-8E6 mAb was designated CD3a by the Second International Swine CD Workshop.

561485 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
561485 Rev.1
Citations & References
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Development References (2)

  1. Pescovitz MD, Book BK, Aasted B. Analyses of monoclonal antibodies reacting with porcine CD3: results from the Second International Swine CD Workshop. Vet Immunol Immunopathol. 1998; 60(3-4):261-268. (Clone-specific). View Reference
  2. Pescovitz MD, Book BK, Aasted B. Summary of workshop findings for antibodies reacting with porcine T-cells and activation antigens: results from the Second International Swine CD Workshop. Vet Immunol Immunopathol. 1998; 60(3-4):251-260. (Clone-specific). View Reference
561485 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.