The 34-5-8S antibody recognizes an epitope on the N-terminal domains, α1 and α2, of the H-2Dd. The mAb identifies a conformationally sensitive epitope of H-2Dd associated with β2 microglobulin; it fails to react with free H-2D[d] α chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. Reactivity with other haplotypes (e.g., f, k, p, r) has not been observed. mAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, C+, F+, G2+, or I+ NK cells or transfectants.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.