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PE Mouse Anti-Human Ig κ Light Chain
PE Mouse Anti-Human Ig κ Light Chain

Multicolor flow cytometric analysis of Ig κ Light Chain expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells (PBMC) were incubated in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody (Cat. No. 555415/561742) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; Left Panel) or PE Mouse Anti-Human Ig κ Light Chain antibody (Cat. No. 555792/562052; Right Panel). The bivariate pseudocolor density plots showing the correlated expression of Ig κ Light Chain (or Ig isotype control staining) versus CD19 were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis was performed using a BD™ LSR II Flow Cytometer System and FloJo™ software.

PE Mouse Anti-Human Ig κ Light Chain

Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)

Multicolor flow cytometric analysis of Ig κ Light Chain expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells (PBMC) were incubated in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody (Cat. No. 555415/561742) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; Left Panel) or PE Mouse Anti-Human Ig κ Light Chain antibody (Cat. No. 555792/562052; Right Panel). The bivariate pseudocolor density plots showing the correlated expression of Ig κ Light Chain (or Ig isotype control staining) versus CD19 were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis was performed using a BD™ LSR II Flow Cytometer System and FloJo™ software.

Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)

Product Details
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BD Pharmingen™
Ig, kappa Light Chain; IGKC; HCAK1; Km; Ig kappa chain C region
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
20 µl
3514
AB_396127
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
555792 Rev. 8
Antibody Details
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G20-193

The G20-193 monoclonal antibody specifically binds to human immunoglobulin light chain, kappa (κ). It does not react with human immunoglobulin λ light chains or human immunoglobulin heavy chains.

555792 Rev. 8
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
555792 Rev.8
Citations & References
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Development References (3)

  1. Cary SP, Lee J, Wagenknecht R, Silverman GJ. Characterization of superantigen-induced clonal deletion with a novel clan III-restricted avian monoclonal antibody: exploiting evolutionary distance to create antibodies specific for a conserved VH region surface. J Immunol. 2000; 164(9):4730-4741. (Clone-specific: Flow cytometry). View Reference
  2. Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-1139. (Biology). View Reference
  3. Widhopf GF, 2nd, Brinson DC, Kipps TJ, Tighe H. Transgenic expression of a human polyreactive Ig expressed in chronic lymphocytic leukemia generates memory-type B cells that respond to nonspecific immune activation. J Immunol. 2004; 172(4):2092-2099. (Biology: Flow cytometry). View Reference
555792 Rev. 8

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.