Two-parameter flow cytometric analysis of FcεR1α expression on human peripheral blood leucocyte populations. Human whole blood was stained with either PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; Left Plot) or PE Mouse Anti-Human FcεR1α antibody (Cat. No. 566607/566608; Right Plot) at 1 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric pseudo color plots showing the correlated expression of FcεR1α (or Ig Isotype control staining) versus side light-scatter signals (SSC-A) were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD FACSCelesta™ Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Two-color flow cytometric analysis of FcεR1α expression on human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD203c antibody (Cat. No. 562973) and either PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; Left Plot) or PE Mouse Anti-Human FcεR1α antibody (Cat. No. 566607/566608; Right Plot) at 1 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric pseudo color plots showing the correlated expression of CD203c versus FcεR1α (or Ig Isotype control staining) were derived from gated events with the light scattering characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCelesta™ Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
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Two-parameter flow cytometric analysis of FcεR1α expression on human peripheral blood leucocyte populations. Human whole blood was stained with either PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; Left Plot) or PE Mouse Anti-Human FcεR1α antibody (Cat. No. 566607/566608; Right Plot) at 1 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric pseudo color plots showing the correlated expression of FcεR1α (or Ig Isotype control staining) versus side light-scatter signals (SSC-A) were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD FACSCelesta™ Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Two-color flow cytometric analysis of FcεR1α expression on human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD203c antibody (Cat. No. 562973) and either PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; Left Plot) or PE Mouse Anti-Human FcεR1α antibody (Cat. No. 566607/566608; Right Plot) at 1 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric pseudo color plots showing the correlated expression of CD203c versus FcεR1α (or Ig Isotype control staining) were derived from gated events with the light scattering characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCelesta™ Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
BD Pharmingen™
FCE1A; FCER1A; Fc-epsilon RI-alpha; FcERI; FceR1a; FcεRI
Human (QC Testing)
Mouse IgG2b, κ
Recombinant Extracellular Domain of FcεR1α
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2744475
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
PE Mouse IgG2b, κ Isotype Control RUO
Size
0.1 mg
Cat No.
555058
APC Mouse anti-Human CD203c RUO
Size
50 Tests
Cat No.
562973
566607 Rev. 2
Antibody Details
AER-37
The AER-37 (CRA-1) monoclonal antibody specifically recognizes FcεRIα which is also known as the high affinity immunoglobulin epsilon receptor subunit alpha (Fc-epsilon RI-alpha). FcεRIα is a type I transmembrane glycoprotein that is encoded by FCER1A (Fc fragment of IgE receptor Ia) which belongs to the Ig gene superfamily. FcεRIα serves as the binding subunit for the Fc region of IgE. It forms part of a heterotetrameric, high-affinity IgE Fc receptor (FceR1/FcεR1) that includes signal transducing subunits, one β-chain (FcεRIβ encoded by MS4A2) and two disulfide-linked γ-subunits (FcεRIγ encoded by FCER1G). FcεRIα is normally expressed on basophils and mast cells and can also be expressed on some monocytes, Langerhans cells, dendritic cells, and eosinophils from allergic donors. FcεRIα plays a major role in allergic responses and in the presentation of allergens to the immune system. The AER-37 antibody reportedly does not compete with IgE for FceR1 binding.
566607 Rev. 2
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566607 Rev.2
Citations & References
Development References (6)
-
Cheng YX, Foster B, Holland SM, et al. CD2 identifies a monocyte subpopulation with immunoglobulin E-dependent, high-level expression of Fc epsilon RI.. Clin Exp Allergy. 2006; 36(11):1436-45. (Clone-specific: Flow cytometry). View Reference
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Foster B, Metcalfe DD, Prussin C. Human dendritic cell 1 and dendritic cell 2 subsets express FcepsilonRI: correlation with serum IgE and allergic asthma.. J Allergy Clin Immunol. 2003; 112(6):1132-8. (Clone-specific: Flow cytometry). View Reference
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Gounni AS, Lamkhioued B, Ochiai K, et al. High-affinity IgE receptor on eosinophils is involved in defence against parasites. Nature. 1994; 367(6459):183-186. (Biology). View Reference
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Jürgens M, Wollenberg A, Hanau D, de la Salle H, Bieber T. Activation of human epidermal Langerhans cells by engagement of the high affinity receptor for IgE, Fc epsilon RI.. J Immunol. 1995; 155(11):5184-9. (Biology). View Reference
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Maurer D, Fiebiger S, Ebner C, et al. Peripheral blood dendritic cells express Fc epsilon RI as a complex composed of Fc epsilon RI alpha- and Fc epsilon RI gamma-chains and can use this receptor for IgE-mediated allergen presentation.. J Immunol. 1996; 157(2):607-16. (Biology). View Reference
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Takai T, Yuuki T, Iwamoto-Yasue N, Okumura K, Ra C. Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions.. Biosci Biotechnol Biochem. 2000; 64(9):1856-67. (Immunogen: Flow cytometry, Western blot). View Reference
566607 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
