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PE Mouse Anti-Human CD280 (MRC2)
PE Mouse Anti-Human CD280 (MRC2)

Flow cytometric analysis of CD280 (MRC2) expression on MG-63 cells. Cells from the human MG-63 (Bone Osteosarcoma, ATCC CRL-1427) cell line were stained with PE Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or PE Mouse Anti-Human CD280 (MRC2) antibody (Cat. No. 566817; solid line histogram) at 0.5 μg/test. The fluorescence histogram showing CD280 (MRC2) expression (or Ig Isotype control staining) was derived from gated events with the light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Flow cytometric analysis of CD280 (MRC2) expression on MG-63 cells. Cells from the human MG-63 (Bone Osteosarcoma, ATCC CRL-1427) cell line were stained with PE Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or PE Mouse Anti-Human CD280 (MRC2) antibody (Cat. No. 566817; solid line histogram) at 0.5 μg/test. The fluorescence histogram showing CD280 (MRC2) expression (or Ig Isotype control staining) was derived from gated events with the light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Product Details
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BD Pharmingen™
MRC2; CLEC13E; endocytic receptor 180; ENDO180; UPARAP
Human (QC Testing)
Mouse IgG1, κ
AG1523 Human Fibroblasts
Flow cytometry (Routinely Tested)
0.2 mg/ml
VIII 80324
AB_2869884
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566817 Rev. 1
Antibody Details
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E1/183

The E1/183 monoclonal antibody specifically recognizes CD280 which is also known as C-type lectin domain family 13 member E (CLEC13E), C-type mannose receptor 2, Endocytic receptor 180 (ENDO180), Urokinase-type plasminogen activator receptor-associated protein (UPARAP), or TEM22. CD280 (MRC2) is a ~180 kDa single-pass type I transmembrane glycoprotein that is encoded by MRC2 (Mannose receptor C type 2). This multifunctional adhesion receptor is comprised of an extracellular region that contains an N-terminal cysteine-rich domain, a fibronectin type II domain, and eight consecutive C-type lectin domains (CTLD1-8) which are followed by a transmembrane region and a small cytoplasmic tail. CD280 (MRC2) is variably expressed on myeloid progenitor cells, macrophages, dendritic cells, endothelial cells, fibroblasts, and mesenchymal cells. CD280 (MRC2) can serve as a recycling endocytic receptor involved in the uptake and degradation of extracellular matrix (ECM) proteins including collagens as well as a pattern recognition receptor (PRR) for microbial pathogens. CD280 (MRC2) may also play roles in cell migration and tumor cell metastasis.

        

566817 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566817 Rev.1
Citations & References
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Development References (7)

  1. Buckley CD, Halder S, Hardie D, et al. Report on antibodies submitted to the stromal cell section of HLDA8.. Cell Immunol. 236(1-2):29-41. (Clone-specific: Flow cytometry). View Reference
  2. Howard MJ, Isacke CM. The C-type lectin receptor Endo180 displays internalization and recycling properties distinct from other members of the mannose receptor family. J Biol Chem. 2002; 277(35):32320-32331. (Biology). View Reference
  3. Isacke CM, van der Geer P, Hunter T, Trowbridge IS. p180, a novel recycling transmembrane glycoprotein with restricted cell type expression. Mol Cell Biol. 1990; 10(6):2606-2618. (Immunogen: Immunofluorescence, Western blot). View Reference
  4. Killick KE, Ni Cheallaigh C, O'Farrelly C, Hokamp K, MacHugh DE, Harris J. Receptor-mediated recognition of mycobacterial pathogens. Cell Microbiol. 2013; 15(9):1484-1495. (Biology). View Reference
  5. Lundberg K, Rydnert F, Greiff L, Lindstedt M. Human blood dendritic cell subsets exhibit discriminative pattern recognition receptor profiles. Immunology. 2014; 142(2):279-288. (Biology). View Reference
  6. Sheikh H, Yarwood H, Ashworth A, Isacke CM. Endo180, an endocytic recycling glycoprotein related to the macrophage mannose receptor is expressed on fibroblasts, endothelial cells and macrophages and functions as a lectin receptor. J Cell Sci. 2000; 113(6):1021-1032. (Clone-specific: Immunofluorescence). View Reference
  7. Wienke D, MacFadyen JR, Isacke CM. Identification and characterization of the endocytic transmembrane glycoprotein Endo180 as a novel collagen receptor. Molec Biol Cell. 2003; 14(9):3592-3604. (Clone-specific: Flow cytometry). View Reference
View All (7) View Less
566817 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.